Transgenic cotton seed purity identification method

Abstract

The present invention provides a transgenic cotton (including hybrid cotton) seed purity identification method. Said method incldues the following steps: using aminoglycoside antibiotics (kanamycin, etc) to prepare seed-soaking aqueous solution with proper concentration, soaking seed, using fresh water sand containing no salt content as germinating bed, sowing, then retaining required temp. and humidity required for germination, after about 7 days the cotton seedling cotyledon is completely patulous, investigating cotton seedling and counting, according to the yellow spot rate identifying purity of seed. It is simple, has no need of disinfecting cotton seed, has no need of making sterile operation and can directly use running water to prepare seed-soaking solution.

Description

technical field [0001] The invention relates to a method for identifying the purity of transgenic crop seeds, in particular to a method for identifying the purity of transgenic cotton seeds, which belongs to the field of agricultural science and technology. Background technique [0002] Transgenic insect-resistant cotton has increasingly attracted scientific research work in various countries due to its many functions in curbing cotton insect damage, saving agricultural costs, reducing labor intensity, reducing environmental pollution caused by spraying pesticides, and promoting the improvement of farmland micro and macro environments. The attention of the researchers [1, 2, 3]. Bt cotton is one of the crops currently approved for commercial production in China. However, there is a lack of rapid and effective purity identification methods in the promotion of Bt cotton, and non-Bt cotton seeds are mixed with Bt cotton, which will make the resistance problem unpredictable and...

AI Technical Summary

Problems solved by technology

However, this method has the following disadvantages: ① The requirements for the test facilities are relatively high, specific tissue culture media need to be prepared, the aseptic operation conditions are strict, and it is difficult to realize the simple, fast, efficient, and universalizable operation required for the purity detection of crop varieties (2000)[5] conducted a confirmatory test using the method reported by Li Yanmo, and found that the method would seriously inhibit the growth of radicle and root of transgenic cotton seedlings, Adverse effects on the normal growth and development of cotton seedlings

[0005] In addition, Ma Lihua et al. (2000) [6], Zhu Jianbo et al. (2000) [5], Zhou Yu (2000) et al. [7] reported a field rapid detection method for transgenic Bt insect-resistant cotton using kanamycin-coated leaves. However, these methods can only be used for the breeding screening of field Bt cotton varieties or the purification of cotton seed Bt resistance genes during the breeding process, and cannot be directly used for the rapid identification of seed purity in the sales process of transgenic Bt cotton