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Collagen used for deep-sea fish skin tissue engineering and its preparing process

A deep-sea fish skin and collagen technology, applied in the field of fish skin collagen, can solve the problems of limited collagen application and low degree of collagen purification, and achieve the effects of high purity, simple method and good biocompatibility

Inactive Publication Date: 2002-12-04
SHANGHAI INST OF ORGANIC CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the low degree of purification of collagen also limits the application of the prepared collagen

Method used

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  • Collagen used for deep-sea fish skin tissue engineering and its preparing process
  • Collagen used for deep-sea fish skin tissue engineering and its preparing process
  • Collagen used for deep-sea fish skin tissue engineering and its preparing process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: preparation of fish skin collagen

[0025] 1. Clean the fish skin Soak 1 kg of frozen salmon skin in tap water at 4°C and rinse it clean, remove scales and subcutaneous tissue until there is no other solid matter in the soaking solution except the whole fish skin, and then use it Soak in deionized water and rinse 5 times.

[0026] 2. Alkaline treatment of fish skin with 5% Na 2 CO 3 Soak 10 liters of solution at 4°C and soak (1) the fish skin after treatment for 1 week, and replace it with 3% Na every day. 2 CO 3 solution. The fish skin after the alkali treatment is fully cleaned with deionized water until the pH is about 7.

[0027] 3. Restricted enzymatic hydrolysis of fish skin Dissolve the fish skin treated in (2) in 5 liters of 1.0 M acetate buffer, add 1.0% trypsin, enzymolyze at 4° C. for 1 day, and filter with gauze. The residue was subjected to restriction enzymatic hydrolysis again, and the steps were the same as above, and the ...

Embodiment 2

[0030] Embodiment 2: preparation of fish skin collagen

[0031] 1. Clean the fish skin. Soak 1 kg of frozen salmon skin in tap water at 1-10°C and rinse it clean. Remove scales and subcutaneous tissue until there is no other solid matter in the soaking solution except the whole fish skin. Soak and rinse with deionized water 5 times.

[0032] 2. Alkaline treatment of fish skin with 1% Ca(OH) 2Soak 10 liters of solution at 1-10°C (1) for 1 week to treat fish skin, and replace 1% Ca(OH) once a day 2 solution. The fish skin after the alkali treatment is fully cleaned with deionized water until the pH is about 7.

[0033] 3. Restricted enzymatic hydrolysis of fish skin Dissolve the fish skin treated in (2) in 5 liters of 1.0M acetate buffer, add 1.0% pepsin, and enzymatically hydrolyze it at 1-10°C for 2 days. filter. The residue was subjected to restriction enzymatic hydrolysis again, and the steps were the same as above, and the two filtrates were combined.

[0...

Embodiment 3

[0035] Embodiment 3: preparation of salted fish skin collagen

[0036] 1. Clean the fish skin Soak 1 kg of frozen salmon skin in tap water at 5°C and rinse it clean, remove scales and subcutaneous tissue until there is no other solid matter in the soaking solution except the whole fish skin, and then use it Soak in deionized water and rinse 5 times.

[0037] 2. Alkali treatment of fish skin Fish skin was salted with NaCl (1:1 w / w) for 7 days. The fish skin after the alkali treatment is fully cleaned with deionized water until the pH is about 7.

[0038] 3. Restricted enzymatic hydrolysis of fish skin Dissolve the fish skin treated in (2) in 5 liters of 1.0 M acetate buffer, add 2.0% pepsin, enzymolyze at 5° C. for 2 days, and filter with gauze. The residue was subjected to restriction enzymatic hydrolysis again, and the steps were the same as above, and the two filtrates were combined.

[0039] 4. Purify collagen. Centrifuge the above filtrate at 10,000rpm at l...

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Abstract

A process for preparing a collagen with high biocompatibility from the skin of deep-sea fish includes treating the skin in alkali solution, restrictive enzymolysis and purifying supernatant. The resultant collagen features high purity, biodegradability and cell compatibility.

Description

technical field [0001] The invention relates to a collagen for deep-sea fish skin tissue engineering and a preparation method. Use deep-sea fish skin as raw material to extract collagen for the preparation of biological substitutes. Specifically, the present invention relates to treating fish skin with alkaline solution, then treating the above fish skin with a restricted enzymatic hydrolysis method, and purifying the enzymatic hydrolysis supernatant. Degradation of biocompatible fish skin collagen. Background technique [0002] Collagen is a structural protein that has a variety of biological functions. Collagen is the support and filler of tissues, actively participates in cell migration, differentiation and proliferation, and is related to wound repair and embryonic development. Biological substitutes prepared from collagen have achieved good results in animal experiments and clinical experiments. [0003] At present, a number of biological products with collagen as th...

Claims

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Application Information

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IPC IPC(8): C12P21/02
Inventor 王瑾晔董健
Owner SHANGHAI INST OF ORGANIC CHEMISTRY - CHINESE ACAD OF SCI
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