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Limonium suffruticosum test tube rapid breeding method for factory

An industrialized, blood-tonifying grass technology, applied in gardening methods, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of incomplete flower ears, uneven plant growth, low rooting rate, etc., and achieve plant growth. The effect of good, full and full flower ears, and fast rooting of young shoots

Inactive Publication Date: 2007-06-06
云南省香料研究开发中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yet the Limonium Limonium tissue culture seedling that supplies on the market still has the following problems: - be that the rooting rate is low, directly affects the transplanting survival rate, not only can increase the planting cost, and can miss the best planting opportunity, brings the grower large economic loss
The second is that most of the tissue culture seedlings have clustered seedlings. When transplanting to the field for planting, the clustered seedlings will compete with the main seedlings for nutrients, resulting in quality problems such as uneven plant growth and insufficient flower spikes.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Explant culture: select the 8cm flower buds extracted from the base, remove the flower buds at the top, cut into 0.8cm stems containing axillary buds, and inoculate the stems in the following explant culture medium after routine sterilization:

[0037] MS Basic Medium

[0038] 6-Benzylaminopurine (6-BA) 1.5mg / L

[0039] Naphthaleneacetic acid (NAA) 0.3mg / L

[0040] Sucrose 30000mg / L

[0041] Agar powder 7000mg / L

[0042] pH 5.8

[0043] Cultivate for 6 weeks, control the temperature at 15°C, light intensity 1000 lux, and light for 15 hours a day;

[0044] 2. Subculture: Screen the robust buds cultured by explants and transfer them to the following subculture medium:

[0045] MS Basic Medium

[0046] 6-Benzylaminopurine (6-BA) 0.6mg / L

[0047] Cultivate for 4 weeks to make it grow into buds whose outer leaves all contain axillary buds;

[0048] 3. Cut the buds: Select the axillary buds wrapped in the leaves, and cut the buds horizontally at the base of the axi...

Embodiment 2

[0059] 1. Explant culture: select the 12cm flower buds extracted from the base, remove the flower buds at the top, cut into 1.2cm stems containing axillary buds, and inoculate the stems in the following explant culture medium after routine sterilization:

[0060] MS Basic Medium

[0061]6-Benzylaminopurine (6-BA) 0.3mg / L

[0062] Sucrose 30000mg / L

[0063] Agar powder 7000mg / L

[0064] pH 5.4

[0065] Cultured for 4 weeks, the temperature was controlled at 28 °C, the light intensity was 800 lux, and the light was 12 hours a day;

[0066] 2. Subculture: Screen the robust buds cultivated by explants and transfer them to the following subculture medium:

[0067] MS basic medium

[0068] 6-Benzylaminopurine (6-BA) 0.2mg / L

[0069] Cultivated for 3 weeks to grow into buds whose outer leaves all contain axillary buds;

[0070] 3. Cutting buds: Select the axillary buds wrapped in the leaves, and cut the buds horizontally at the base of the axillary buds. The cut buds have a fl...

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PUM

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Abstract

The present invention provides an industrial method in text tube for fast cultivation of limonium, comprising external cultivation, subculture, cultivation of root and transitional cultivation, characterized in that the axillary buds packed in the leaf are selected and cut horizontally at its base, with the cut buds transferred to the root culture medium. The cultivation is carried out 4-5 days at room temperature with dark and light treatment of 12-15 hours each day for 4-5 days when the original base of roots have grown around the cut of buds, and transplanting is carried out when radial roots 4-5 mm long are all around the base of buds and 4-6 leaves have grown in the upper part of the buds. The invention can accelerate the growth of buds and have a high rate of buds which grow roots, whose survival rate can reach 100 % after transplanted, and can produce essential economic benefit and meet the limonium demand of market.

Description

technical field [0001] The invention relates to a tissue culture seedling propagation method of a plant flower, Lemonaceae, and belongs to the technical field of tissue culture of plant seedlings. Background technique [0002] Limonium, commonly known as forget-me-not, is a perennial or biennial herbaceous plant of the genus Limonaceae (or Pinaceae), and is a famous ornamental flower. In recent years, with the development of my country's economy and the improvement of people's living standards, Limonium Limonium has become an important cut flower species in my country's flower market. Yet the Limonium Limonium tissue culture seedling that supplies on the market still has the following problems: - be that the rooting rate is low, directly affects the transplanting survival rate, not only can increase the planting cost, and can miss the best planting opportunity, brings the grower greater economic loss. The second is that most of the tissue culture seedlings have clustered s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 易清元
Owner 云南省香料研究开发中心
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