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Process for biologically synthesizing bastardtoadflex extract

A technology of biosynthesis and thymosin, applied in plant cells, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problem of low yield and achieve the goal of protecting biodiversity and protecting the ecological balance of nature Effect

Inactive Publication Date: 2004-11-17
王志强 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to meet the market demand, many places have put forward plans for artificial cultivation, but due to the extremely low yield (about 5-7 kg per mu per year), it can only be widely planted and poorly harvested, and there is bound to be a situation of competition with grain for land.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0012] 1. Preparation of solid medium:

[0013] 6.7-V, 1~10% host extract, 1% sucrose (carbon source), 20mg / L NH 2 NO 3 (Nitrogen source), 2mg / L 2.4-D, 2mg / L NAA, 1mg / L KT, 0.8% agar (coagulant), pH5.3~5.8.

[0014] 2. Preparation of fluid medium:

[0015] 6.7-V, 1~10% host extract, 0.6% sucrose, 0.4% glucuronic acid, 2mg / L 2.4-D, 0.5mmol / L NAA, 0.5mmol / L Met, 0.05mmol / L cysteine ​​( Cys) or vitamin C, pH5.2~6.5.

[0016] 3. Subculture:

[0017] Take the whole herb of Thyme chinensis, wash it with tap water, wash it with sterile water after sterilization, cut it into 2-3mm long fragments, put it into a culture tank, put it in a solid medium, and carry out subsequent cultivation at a temperature of 25-28°C. subculture. Supplement 1-2 mg / L casein to the solid medium every week during subculture. After subculture of 6-7 generations (about 2-3 days per generation) or more, the tissues are dispersed, and then fermented.

[0018] 4. Fermentation culture:

[0019] After subc...

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Abstract

A process for biological synthesizing bastardtoadflex extract is characterized by that the sterilized bastardtoadflex fragment goes through secondary culture and fermentation culture in the culture medium which is prepared from standard culture liquid, liquid host extract, carbon source, nitrogen source and auxin. Its advantages are need of less fragments, short peroid and high output increased by 30 times.

Description

1. Technical field [0001] The invention relates to a method for rapid propagation of plant cells, in particular to a method for biosynthesis of thymosin. 2. Background technology [0002] Thyme grass (Thesium chinese Turcz) is the whole herb of the sandalwood spice (Sanlatacesae) plant. [0003] Thyme is a Chinese herbal medicine known for its broad-spectrum antibacterial and anti-inflammatory properties. [0004] The antitussive lotion disclosed by CN1104110A and CN1165691A and the ointment for relieving cough and relieving asthma all use thyme grass. At present, the call for the modernization of traditional Chinese medicine is getting louder and louder. In addition, as people continue to "return to nature" in their own survival activities, the Chinese herbal medicine market continues to expand, and thyme grass, which cannot be replaced by other Chinese herbal medicines, has become a leader in the market competition. However, thyme is a semi-parasitic plant with few wild...

Claims

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Application Information

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IPC IPC(8): C12N5/04
Inventor 罗曼王志强蒋立科
Owner 王志强
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