Biological assay method of rogal jelly and freeze-dried powder activity

A bioassay and royal jelly technology, applied in measuring devices, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve the problem that the detection method cannot fully reflect the activity of royal jelly and freeze-dried powder, that is, the nutritional status, and achieve the convenience of implementation Effect

Inactive Publication Date: 2004-09-01
NANJING LAOSHAN PHARMA
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is that the existing detection methods cannot fully reflect the activity of royal jelly and freeze-dried powder, that is, the nutritional status

Method used

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Examples

Experimental program
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Embodiment Construction

[0015] The following details of the present invention are specifically described below:

[0016] 1. Preparation of royal jelly feed: Royal jelly should be thawed, freeze-dried powder is converted into royal jelly with sterile saline, and ground to make it evenly dispersed.

[0017] 2. The larvae should be selected from the disease-free bee colony to be one-day-old larvae with the same size and growth.

[0018] 3. Strictly disinfect all utensils after washing. The specific method is: before feeding the larvae each time, clean the mortar, incubator, and insect transfer tool with clean water, and then sterilize the equipment with high-pressure steam. It is not allowed to use high-pressure and neatly sterilized appliances. For example, insect transfer needles can be soaked in distilled water for 10 minutes, and then rinsed with distilled water.

[0019] 4. Spread the feed evenly into the incubator, and put it into the cotton ball soaked in water, keep the humidity above 90%, put...

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Abstract

The royal jelly and freeze-dried powder activity detection method includes the following steps: 1. preparing royal jelly feed; 2. selecting health larva with age in one day from hive; 3. uniformly spreading feed in culture container, relaining humidity at above 90%, placing in culture box, controlling temp. at about 34 deg.C; 4. transferring 30 larvae in culture container, constant temp. culture; 5. replacing feed every day, and weighing larvae; and 6. after 7 days one-by-one weight larvae and making statistic analysis of the results.

Description

technical field [0001] The invention relates to a method for detecting the activity of royal jelly and freeze-dried powder. Background technique [0002] Royal jelly and freeze-dried powder have miraculous effects, and its active ingredients are composed of various substances. The methods currently used to detect the activity of royal jelly and freeze-dried powder are mainly chemical analysis methods, such as RP-HPLC, high performance liquid chromatography, gas chromatography-mass spectrometry and other methods. The above methods can only detect one or several active ingredients in royal jelly or freeze-dried powder, and cannot fully reflect their biological activity and nutritional value. For example: At present, most manufacturers use the content of royal jelly acid as the standard to measure the quality of royal jelly, but the chemical properties of royal jelly acid are relatively stable, even after high temperature treatment, the content wi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/644G01N5/00G01N33/00
Inventor 王加聪陈坤李晓栋裴余一吴黎明张复兴邱建勇
Owner NANJING LAOSHAN PHARMA
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