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Microalgae biological compound liquid fertilizer as well as preparation method and application thereof

A technology of microalgae biology and composite liquid, applied in the direction of microorganism-based methods, biochemical equipment and methods, botany equipment and methods, etc., to achieve good release, good fertility, and improved yield and quality

Pending Publication Date: 2022-08-05
INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microbial fertilizers, also known as biological fertilizers and bacterial fertilizers, are a type of microbial living products that use microbial life activities and their products to cause crops to obtain specific fertilizer effects. Research on the microalgae biological compound liquid fertilizer for crops, especially the effect of the microalgae biological compound liquid fertilizer in the cucumber and tomato rotation system in the facility

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  • Microalgae biological compound liquid fertilizer as well as preparation method and application thereof
  • Microalgae biological compound liquid fertilizer as well as preparation method and application thereof
  • Microalgae biological compound liquid fertilizer as well as preparation method and application thereof

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preparation example Construction

[0026] The present invention also provides a method for preparing the above-mentioned microalgae biological compound liquid fertilizer, comprising the following steps:

[0027] After culturing the photosynthetic green algae and Chlorella pyrenoidosa respectively, the photosynthetic green algae liquid and the Chlorella pyrenoidosa algal liquid are obtained;

[0028] Mixing the photosynthetic trophic green algae liquid and Chlorella pyrenoidosa algae liquid, and taking 1 / 3-1 / 2 mass of the mixed algal liquid for precipitation to obtain mixed algal sludge;

[0029] The mixed algal mud is mixed with the PBS solution, and ultrasonically crushed to obtain a mixed algal mud treatment solution;

[0030] Mixing the remaining mixed algal liquid, mixed algal mud treatment liquid, Brevibacterium lateralis and Streptomyces lividans to obtain microalgae biological compound liquid fertilizer.

[0031] In the present invention, the photosynthetic green algae and Chlorella pyrenoidosa are resp...

Embodiment 1

[0039] A microalgae biological compound liquid fertilizer is 30 parts of Chlorella pyrenoidosa, 25 parts of photosynthetic trophic green algae, 10 parts of Brevibacillus marinus sporogenes AMCC 100018, and 10 parts of Streptomyces flavus AMCC 400001.

[0040] The preparation method of above-mentioned microalgae biological compound liquid fertilizer, comprises the following steps:

[0041](1) after culturing the photosynthetic green algae and Chlorella pyrenoidosa respectively, obtain the photosynthetic green algae liquid and Chlorella pyrenoidea algal liquid;

[0042] (2) mixing the photosynthetic nutrient green algae liquid and Chlorella pyrenoidosa algae liquid uniformly, taking 1 / 2 mass of the mixed algae liquid and centrifuging it at 3500rpm for 5min to obtain mixed algal sludge;

[0043] (3) Wash the mixed algal mud with PBS solution twice, after washing, add PBS solution and mix until the density of the mixed algal mud is 5×10 6 pcs / mL, ultrasonically crushed under the ...

Embodiment 2

[0046] A microalgae biological compound liquid fertilizer is: 25 parts of Chlorella pyrenoidosa, 30 parts of photosynthetic trophic green algae, 15 parts of Brevibacillus lateralis sp. AMCC 100018, and 5 parts of Streptomyces flavonoids AMCC 400001.

[0047] The preparation method of above-mentioned microalgae biological compound liquid fertilizer, comprises the following steps:

[0048] (1) after culturing the photosynthetic green algae and Chlorella pyrenoidosa respectively, obtain the photosynthetic green algae liquid and Chlorella pyrenoidea algal liquid;

[0049] (2) mixing the photosynthetic nutrient green algae liquid and Chlorella pyrenoidosa algae liquid uniformly, taking 1 / 3 of the mass of the mixed algae liquid and centrifuging it at 4000rpm for 4min to obtain mixed algal sludge;

[0050] (3) Wash the mixed algal mud with PBS solution once, after washing, add PBS solution and mix until the density of the mixed algal mud is 8×10 6 pcs / mL, ultrasonically crushed unde...

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Abstract

The invention provides a microalgae biological compound liquid fertilizer as well as a preparation method and application thereof, and belongs to the technical field of biological fertilizers, the microalgae biological compound liquid fertilizer comprises the following components in parts by weight: 25-45 parts of chlorella pyrenoidosa, 15-30 parts of photosynthetic nutrient green algae, 5-15 parts of marine brevibacillus laterosporus and 5-15 parts of streptomyces microflavus. The chlorella pyrenoidosa, the photosynthetic nutrient green algae, the marine brevibacillus laterosporus and the streptomyces microflavus are added to achieve a synergistic effect, so that nutritional ingredients such as organic matters, nitrogen and phosphorus in soil are effectively improved, the yield and quality of vegetables are remarkably improved, and meanwhile, the microalgae biological compound liquid fertilizer is green, safe and high in utilization rate.

Description

technical field [0001] The invention relates to the technical field of biological fertilizers, in particular to a microalgae biological compound liquid fertilizer and a preparation method and application thereof. Background technique [0002] Cucumber is a high-yielding vegetable with fast growth, many fruits, long fruiting period, and large consumption of nutrients. Cucumber has a large demand for the three elements of nitrogen, phosphorus and potassium. The distribution is shallow in the middle, it is difficult to use the water and nutrients below the root layer, and the root system has weak fertility tolerance. Tomato is a vegetable with continuous flowering and fruiting. It has a long growth period and high yield. It needs to absorb a lot of nutrients from the soil throughout the growth period, mainly nitrogen, phosphorus and potassium. It has strong fertilizer tolerance and requires a large amount of fertilizer. To sum up, cucumber tomato is a typical high-input, high-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/20A01G22/00A01G22/05C12R1/01C12R1/465C12R1/89
CPCC12N1/12C12N1/20A01G22/00A01G22/05Y02W30/40
Inventor 王丽英蒋龙刚任燕利陈莉史建硕郭丽李若楠邢向远宋晓陈丹李建芬
Owner INST OF AGRI RESOURCES & ENVIRONMENT HEBEI ACADEMY OF AGRI & FORESTRY SCI
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