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Preparation method of high-activity nitrifying bacteria biological membrane for aquaculture water treatment

An aquaculture, nitrifying bacteria technology, applied in biological water/sewage treatment, water/sludge/sewage treatment, seawater treatment and other directions, can solve the problem of low ammonia nitrogen removal rate, improve nitrification activity, speed up preparation speed, promote effect of growth

Active Publication Date: 2022-06-28
QINGDAO TECHNOLOGICAL UNIVERSITY
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Problems solved by technology

[0008] Aiming at the problem that ammonia nitrogen, nitrite nitrogen and other pollutants are difficult to remove in aquaculture ponds and the deficiencies in the existing nitrifying bacteria microbial film preparation technology, the present invention proposes a method that promotes the growth of biofilm nitrifying bacteria, has fast preparation speed and high nitrifying activity. A method for preparing a highly active nitrifying bacteria biofilm for aquaculture water treatment to solve the problem of low removal rate of ammonia nitrogen in aquaculture water

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  • Preparation method of high-activity nitrifying bacteria biological membrane for aquaculture water treatment
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  • Preparation method of high-activity nitrifying bacteria biological membrane for aquaculture water treatment

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preparation example Construction

[0033] The preparation method of the nitrifying bacteria biofilm of the present invention specifically includes the following steps.

[0034] (1) Load the mixture of seaside fine sand and aquaculture pond water into the reactor, and the volume of sea sand is 1 / 3 of the volume (mixture) of the reactor.

[0035] (2) Add 300-500 mg of MgSO to every 1000 mL of distilled water 4 ·H 2 O, 20~50mg of CaCl 2 ·H 2 O, 0.2~2.0mg of MnCl 2 ·H 2 O, 0.1 to 0.2 mg of Na 2 MoO 4 ·H 2 O, 0.1~0.2mg CoCl 2 ·H 2 O, 0.1~0.2mg of ZnSO 4 ·H 2 O and 80 to 500 mg of K 2 HPO 4 , mixed evenly, and configured into a nutrient solution.

[0036] Add 1-2% nutrient solution to the reactor mixture to form a mixed solution together with the mixture.

[0037] Elements such as Mg, Ca, Mn, Na, Co, Zn and K, especially Mn, Co and Zn, are necessary trace elements for some efficient nitrifying bacteria species, which can avoid being disadvantaged due to the lack of these trace elements. competitive p...

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Abstract

The invention relates to a preparation method of a high-activity nitrifying bacteria biological membrane for aquaculture water treatment. The preparation method comprises the following steps: (1) adding sea sand into aquaculture pond water; (2) adding a nutrient solution; (3) aerating, and controlling the concentration of dissolved oxygen; (4) adding a ferric hydroxide solution; (5) maintaining the ammonia nitrogen concentration at 2-20 mg / L all the time, and maintaining the pH value at 7-9 all the time; (6) adding a suspended carrier according to the concentration of suspended matters except the sea sand in the mixed solution; (7) adjusting the concentration of dissolved oxygen; (8) stopping aeration and taking out the sea sand when more than 20% of the suspended carrier is turned over along with the water flow; (9) starting aeration and maintaining the dissolved oxygen concentration; and (10) when the ammonia nitrogen cannot be detected, completing the preparation of the biological membrane. According to the method, the fixing speed of the nitrifying bacteria biological membrane is increased, and the nitrifying bacteria biological membrane has efficient nitration reaction activity and can quickly remove ammonia nitrogen and nitrite nitrogen in water.

Description

technical field [0001] The invention relates to a preparation method of a highly active nitrifying bacteria biofilm for treating aquaculture water, and belongs to the technical field of nitrifying bacteria biofilm preparation. Background technique [0002] In the process of aquaculture, ammonia nitrogen and nitrite nitrogen are produced by the manure and residual food of aquatic organisms, which pose a threat to the health of aquatic organisms. For the removal of ammonia nitrogen and nitrite nitrogen in water, the microbial method is an economical, feasible, environmentally friendly and efficient method. [0003] The microbial method uses nitrifying bacteria to remove ammonia nitrogen and nitrite nitrogen in water under aerobic conditions, while nitrifying bacteria, as autotrophic bacteria, have a long generation cycle and require harsh growth environments, so they are often unable to compete with heterotrophic microorganisms. gain an advantage. Therefore, when the ammonia...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/16C02F103/08
CPCC02F3/34C02F2103/08C02F2101/16C02F2101/166C02F2305/06Y02W10/10
Inventor 周小琳李子浩鞠鸿林张源孙鹏成钟宛真刘育铭宫筱彭天瑞高宏宇毕学军程丽华
Owner QINGDAO TECHNOLOGICAL UNIVERSITY
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