Non-symbiotic germination method for seeds of rare and endangered plant calanthe davidiana

Abstract

The invention discloses a non-symbiotic germination method for seeds of rare and endangered plant calanthe davidiana. The method comprises the following steps: disinfection of explants: selecting mature and non-cracked capsules for disinfection; seed germination culture: taking out the seeds, uniformly sowing the seeds on a culture medium, and performing sterile germination under an illumination condition; inducing lateral bud differentiation culture: transferring the small pseudobulb without the leaves into a differential culture medium, and culturing under an illumination condition; rooting and seedling strengthening culture: when the differentiated seedlings grow 1-2 leaves and the length of the leaves is 2-3cm, transferring the seedlings into a rooting and seedling strengthening culture medium for culture; seedling hardening and transplanting: transplanting the tissue culture seedlings into a greenhouse for seedling hardening, taking out, cleaning, disinfecting and soaking; and transplanting the seedlings into a seedling-raising hole tray filled with a substrate. Mature and non-cracked capsules are selected as explants, disinfection, seed germination, lateral bud differentiation, rooting and seedling strengthening, seedling hardening and transplanting are sequentially carried out, culture parameters of all stages are optimized, and a large-scale propagation means and a scientific and effective protection method are established.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and in particular relates to a non-symbiotic germination method for seeds of the rare and endangered plant rhubarb orchid. Background technique [0002] Reproduction can be said to be the most critical part of the entire life history of a species, and it is also an important step in population maintenance and renewal. Research on reproductive biology has always been a popular direction in the study of endangered plants. The history of using orchids in my country can be traced back to BC. However, due to the immaturity and popularization of artificial propagation technology, it is difficult to meet the growing consumption demand of the current market, and the wild resources of many species have been destructively collected. Orchidaceae reproduction has two remarkable characteristics: one is that the seeds of orchidaceae plants are usually very small, and there may be millions of seeds in...

AI Technical Summary

Problems solved by technology

The history of using orchids in my country can be traced back to BC. However, due to the immaturity and popularization of artificial propagation technology, it is difficult to meet the growing consumption demand of the current market, and the wild resources of many species have been destructively collected.

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