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QTL qHD1b for promoting rice heading and application of QTL qHD1b

A rice, RM10390 technology, applied in the fields of molecular biology and plant genetics and breeding, can solve the problem of insufficiently clearly explaining the regulation mechanism of rice heading stage, and achieve the effect of improving traits

Pending Publication Date: 2022-04-15
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these studies are not enough to clearly elucidate the regulation mechanism of rice heading date

Method used

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  • QTL qHD1b for promoting rice heading and application of QTL qHD1b
  • QTL qHD1b for promoting rice heading and application of QTL qHD1b
  • QTL qHD1b for promoting rice heading and application of QTL qHD1b

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0188] Example 1 Phenotype of qHD1b

[0189] Using common wild rice (Oryza rufipogon) as the donor parent, an introgression line was constructed and a new QTL for heading date was identified. Specifically, 14 polymorphic markers were used to construct an introgression line IL7391 ( figure 1 ). Under different conditions, the heading date of IL7391 was earlier than that of the recurrent parent ZH8015. Among them, IL7391 (85.3±3.3d) headed 7.5 days earlier than ZH8015 (92.8±2.3d) under Hangzhou natural long-day (NLD) conditions ( figure 2 A, B); Similarly, under natural short-day (NSD) conditions in Lingshui, it was found that IL7391 (105.4±1.7d) headed 8.8 days earlier than ZH8015 (114.2±1.9d) (Fig. 2B). In the growth incubator under the conditions of artificially controlled long day (CLD) and artificially controlled short day (CSD), the days of heading date of IL7391 were about 13.0 and 18.1 days earlier than that of ZH8015 ( figure 2 B).

Embodiment 2

[0190] Embodiment 2 QTL analysis of heading stage

[0191] In order to analyze the QTL of the heading stage gene region, 150 IL7391 / ZH8015 F 2:3 family lineage. Screening from F 2:3 300 plants sampled in families (2 plants per row). Two QTLs for heading date were detected under NLD, which were qHD1b located on chromosome 1 and qHD7 located on chromosome 7. No QTL for heading date was detected in the other 4 introgression areas ( figure 1 ). The LOD values ​​of qHD1b and qHD7 were 37.99 and 2.8, respectively, and the explained rates of phenotypic variation were 47.7% and 3.8%, respectively. The direction of the additive effect of the two is opposite, qHD1b Oryza rufipogon Alleles lead to early heading in rice (additive effect -3.22), while qHD7 Oryza rufipogon Alleles lead to late heading in rice (additive effect 0.14). Therefore, we decided to focus further analysis on qHD1b, which has a greater influence on the estimation of heading date than qHD7. Thus, the qHD1b ...

Embodiment 3

[0192] Example 3 Separation of NIL (qHD1b) and Fine Mapping of qHD1b

[0193] In order to further locate qHD1b, F 4:5 There are 52320 strains in the family. Using 6 SSR markers located between RM10316 and RM10394, randomly select F 4:5 200 individuals in the family were analyzed for QTL. qHD1b detected 49% of the phenotypic variation. We further investigated whether qHD1b is involved in the photoperiod response in rice. Therefore, we obtained a homozygous allele from the rice F10316-RM10394 region 4:5 NIL(qHD1b) plants were isolated from the population ( figure 1 ). After observing the photoperiod response in the field and in the incubator, it was found that NIL(qHD1b) plants consistently flowered earlier than ZH8015 under four different conditions ( figure 2 C, D). This again shows that qHD1b Oryza rufipogon Alleles promote flowering regardless of day length.

[0194] Meanwhile, we are in F 4:5 2578 very late pumping plants were selected in the population, and qHD...

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Abstract

The invention discloses a QTL qHD1b for promoting heading of rice and application of the QTL qHD1b. A BC5F2: 3 group constructed by backcrossing IL7391 obtained by taking Zhonghui 8015 as a receptor and taking common wild rice as a donor and ZH8015 is taken as a material, and QTL qHD1b influencing the heading stage of the rice is finely positioned in an interval of 112.7-kb between a marker INDEL4 and a marker RM10390. It is found for the first time that QTL qHD1b can influence the heading period of rice under the long and short sunlight conditions, qHD1b further promotes expression of Hd3a, RFT1, OsMADS14 and OsMADS15 by promoting expression of a gene Ehd1, and heading of rice IL7391 is promoted. The QTL qHD1b disclosed by the invention has important significance on molecular marker-assisted selection for cultivating early heading rice varieties, and provides an effective means for improving the heading period characters of rice under long and short sunlight conditions.

Description

technical field [0001] The invention belongs to the fields of molecular biology and plant genetic breeding, and in particular relates to a QTL qHD1b for promoting heading of rice and its application. Background technique [0002] Rice (Oryza sativa L.) is the staple food of more than half of the world's population. Heading date is an important marker for plants to transition from the vegetative stage to the reproductive stage. The flowering process is regulated by various internal and external signals such as photoperiod, temperature and plant hormone levels. The ability of a plant to flower precisely at the appropriate time during its reproduction depends on precise monitoring of seasonal variations in day length and temperature (Brambilla and Fornara 2013; Zhou et al. 2021). Plants integrate cellular timing systems by sensing light signals and circadian clock changes to determine dynamic changes in photoperiod and precisely control flowering time (Wei et al. 2020). For ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11C12N15/29C12N15/113A01H1/04
Inventor 吴玮勋程式华刘嶺曹立勇张迎信占小登杨正福
Owner CHINA NAT RICE RES INST
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