Thermomyces lanuginosus lipase mutant and coding gene

A technology of thermomyces and coding genes, which is applied in genetic engineering, plant genetic improvement, hydrolytic enzymes, etc., can solve the problem that the enzyme activity cannot be effectively guaranteed, and achieve the effect of high enzyme activity

Pending Publication Date: 2022-04-12
NANTONG CHANGYOO PHARMATECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Chinese patent, publication number CN103045559A, discloses thermomyces lanuginosa lipase mutant, coding gene and its application, but its enzyme activity cannot be effectively guaranteed

Method used

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  • Thermomyces lanuginosus lipase mutant and coding gene
  • Thermomyces lanuginosus lipase mutant and coding gene
  • Thermomyces lanuginosus lipase mutant and coding gene

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Experimental program
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Embodiment Construction

[0037] The embodiments of the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0038] In this example, the patent "Thermomyces lanuginosa lipase mutant, coding gene and its application" (Patent No. 201210407833) was used as a positive control to compare the enzyme activity after the point mutation of the present application.

[0039] The peptide sequence of patent 201210407833 is recorded as 7833:

[0040] RPVRRAVPQD LLDQFELFSQ YSAAAYCAAN NHAPVGSDVT CSENVCPEVD AADATFLYSFEDSGLGDVTG LLALDNTNKL IVLSFRGTRS VENWIANLNA DLTEISDICS GCEGHDGFVT SWRSVADTIREQVQNAVNEH PDYRVVFTGH SLGGALATIA AAALRGNGYN IDVFSYGAPR VGNRAFAEFL TAQTGGTLYRITHTNDIVPR LPPRDWGYSH SSPEYWVTSG NDVPVTANDI TVVEGIDSTD GNNQGNIPDI PSHLWYFGPISECD。

[0041] The starting sequence of this example (AF054513.1), the TLL peptide sequence is recorded as AF (MRSSLVLFFV SAWTALA is the signal peptide):

[0042] MRSSLVLFFV SAWTALASPI RREVSQDLFN QFNLFAQYSA AAYCGKNNDA PAGTNITCT...

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Abstract

The invention discloses a thermomyces lanuginosus lipase mutant and a coding gene. The amino acid sequence of the mutant is shown as SEQ ID NO: 2. According to the mutant disclosed by the invention, polar amino acid at a specific position is mutated into non-polar amino acid, site-directed mutagenesis molecular modification of rational design is carried out on existing enzyme molecules, the mutated enzyme molecular structure is not destructively changed, a mutation result is relatively safe, and enough enzyme activity can be ensured or higher enzyme activity can be obtained.

Description

technical field [0001] The present invention relates to the technical field of bioengineering, in particular, to a mutation obtained by performing site-directed mutation on the source of Thermomyces lanuginosus lipase (TLL) gene (GenBank: AF054513.1) bodies and coding genes. Background technique [0002] Thermophilic lipase is a kind of biological enzyme that can maintain catalytic activity under high temperature conditions. It still has good stability under high temperature and can meet the needs of industrial production. Thermophilic lipase mainly comes from microorganisms living under extremely hot conditions. Its reaction conditions are relatively mild, its conversion rate is high, its specificity is strong, it is not easy to produce by-products, and it avoids harmful substances caused by chemical catalysis. Therefore, it is becoming more and more popular. More and more are used in organic synthesis, especially biocatalytic synthesis of optically active compounds, natur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/20C12N15/55
Inventor 许兆青李屹李泽标黄珂顾文超刘平林燕峰
Owner NANTONG CHANGYOO PHARMATECH CO LTD
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