Improved methods for screening, diagnosing and/or monitoring advanced colorectal tumors, advanced adenomas and/or colorectal cancer

A colorectal, tumor technology, applied in the field of cancer diagnosis

Pending Publication Date: 2022-03-18
GOODGUT SL +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, there is evidence that some dietary components metabolized by the gut microbiota, such as red meat, lead to the production of carcinogenic compounds (Joshi et al, 2015)

Method used

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  • Improved methods for screening, diagnosing and/or monitoring advanced colorectal tumors, advanced adenomas and/or colorectal cancer
  • Improved methods for screening, diagnosing and/or monitoring advanced colorectal tumors, advanced adenomas and/or colorectal cancer
  • Improved methods for screening, diagnosing and/or monitoring advanced colorectal tumors, advanced adenomas and/or colorectal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0303] Example 1.- Materials and methods

[0304] research group

[0305] A cohort of 333 consecutive patients with CRC-associated symptoms referred from primary and secondary healthcare to the Complexo Hospitalario de Ourense (Ourense, Spain) was recruited. A diagnostic colonoscopy was performed (Table 1). Exclusion criteria were: (1) asymptomatic subjects undergoing colonoscopy for CRC screening; (2) patients with a history of previous colonic disease undergoing surveillance colonoscopy; (3) patients requiring hospitalization; (4) pre-evaluation Patients whose symptoms have stopped within 3 months; and (5) patients who have received antibiotic treatment within the last month before enrollment. The study protocol was approved by the Biobanco del Complexo Hospitalario Universitario de Vigo (Vigo, Spain). Written informed consent was obtained from all study patients.

[0306] Table 1. Classification of patient characteristics according to colonoscopy diagnosis. Hb: hemoglo...

Embodiment 2

[0331] Example 2.- Fecal biomarkers in tumor progression

[0332] The relative abundance of each bacterial marker was determined for each diagnosis (normal colonoscopy, non-advanced adenoma, advanced adenoma, CRC) ( figure 1 ). Three different butyrate-producing species (B10, B46 and B48) were the most prevalent biomarkers regardless of the colonoscopic diagnosis, with relative abundance values ​​of 20.4%, 19.0% and 20.0%, respectively. GMLL and PTST were significantly more in the CRC population compared to colonoscopically normal individuals (p=0.006 and p<0.001, respectively) or non-advanced adenoma subjects (p=0.047 and p<0.001, respectively). Although there were no significant differences, a trend could be observed for B46, which was more abundant in CRC patients than in advanced adenoma patients (p=0.087). Interestingly, EUB abundance remained constant regardless of tumor status. Comparisons between different CRC stages (0, I, II, III, and IV) did not reveal significan...

Embodiment 3

[0333] Example 3.-GMLL, PTST and BCTF can detect advanced tumor lesions

[0334] The relative abundance of bacterial markers was compared after grouping subjects as follows: (1) normal colonoscopy; (2) tumor (non-advanced adenoma + advanced adenoma + CRC); (3) advanced tumor (advanced adenoma + CRC ); and (4) CRC( figure 2 ). It was found that PTST was highly correlated with tumor lesions (p<0.001). Regarding the detection of advanced neoplastic lesions, GMLL, PTST and BCTF were potential biomarkers for the detection of advanced neoplastic lesions (p=0.006, p<0.001 and p=0.030, respectively). In terms of prevalence, these three opportunistic pathogens were found to be more common in patients with advanced tumors (GMLL, 64.9%; PTST, 58.4%; and BCTF, 44.7%) than in healthy subjects (GMLL, 53.5%; PTST, 26.1%; and BCTF, 29.8%) more.

[0335] Our results clearly demonstrate the presence of bacterial dysbiosis in CRC patients. The bacterial markers studied were categorized acc...

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Abstract

The present invention relates to improved methods of screening, diagnosing and / or monitoring advanced colorectal neoplasms (AN), advanced adenomas (AA) and / or colorectal cancer (CRC), wherein AN comprises CRC and AA. In particular, the methods provide increased specificity due to a reduction in false positive results in Fecal occult blood testing (FOBT) using features based on bacterial markers. The invention further relates to the use of the method in selecting a subject for exploratory testing (e.g., colonoscopy) or for anti-cancer therapy treatment.

Description

technical field [0001] The present invention relates to the field of cancer diagnosis. Specifically, the present invention relates to an improved method for screening, diagnosing and / or monitoring colorectal advanced neoplasia (Advanced neoplasia, AN), advanced adenoma (Advanced adenoma, AA) and / or colorectal cancer (Colorectal cancer, CRC), Among them, AN includes CRC and AA. In particular, the method provides increased specificity due to the reduction of false positive results in Fecal occult blood tests (FOBT) using bacterial marker-based signatures. The invention further relates to the use of said method in selecting a subject for exploratory testing (eg colonoscopy) or for treatment with anti-cancer therapy. Background technique [0002] CRC is the third most common cancer in men and the second most common cancer in women worldwide, and the leading cause of cancer death (Global Cancer Estimated Incidence, Mortality, and Prevalence 2012, http: / / globocan.iarc .fr / Pages...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6886C12Q1/689
CPCC12Q1/689C12Q1/6886G01N33/721C12Q2600/158C12Q1/06C12Q1/6851C12Q1/686
Inventor 马里奥娜·萨拉·佩吉斯杰西·加西亚-吉尔泽维尔·奥尔迪格·曼特玛塔·马拉贡·罗德里格斯萨拉·拉米欧·普约尔
Owner GOODGUT SL
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