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Preparation method and application of anti-inflammatory and kidney-protecting clam peptide

A technology for protecting kidney and clams, which is applied in the field of biomedicine, can solve the problems of little research, no inflammation of clam peptides and protection of kidney function, etc., and achieves the effects of simple process, excellent taste and flavor, and high safety.

Active Publication Date: 2022-03-04
CHENLAND NUTRITIONALS INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research on clam active peptides mostly focuses on biological functions such as lowering blood pressure, lowering blood lipids, and delaying aging. There are not many studies on other aspects of clam active peptides, and there is no clam peptide in alleviating inflammation caused by high blood pressure and protecting kidney function. related reports

Method used

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  • Preparation method and application of anti-inflammatory and kidney-protecting clam peptide
  • Preparation method and application of anti-inflammatory and kidney-protecting clam peptide
  • Preparation method and application of anti-inflammatory and kidney-protecting clam peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 of the present invention discloses a preparation method and application of a clam peptide for anti-inflammatory and kidney-protecting, and the adopted technical scheme is as follows:

[0031] Take the whole meat of Hongdao clams, wash and drain, then homogenize, add a certain amount of deionized water to the clam pulp, so that the final clam meat: water = 1:2 weight ratio. Set the enzymatic hydrolysis temperature to 50°C, add 0.13% of the weight of the clam meat pulp compound protease (the formula is neutral protease: alkaline protease: flavor protease = 2:1:1), enzymatic hydrolysis for 4 hours, centrifuge at 16000r / min, put After the clear liquid is filtered through microfiltration-ultrafiltration-nanofiltration membrane, it is spray-dried to obtain clam peptide.

Embodiment 2

[0033] Example 2 of the present invention discloses a preparation method and application of a clam peptide for anti-inflammatory and kidney-protecting, and the adopted technical scheme is as follows:

[0034] Take the whole meat of Hongdao clams, wash and drain, then homogenate, and add a certain amount of deionized water to the clam pulp, so that the final clam meat:water=1:3 weight ratio. Set the enzymolysis temperature to 50°C, add 0.13% of the weight of the clam pulp compound protease (the formula is neutral protease: alkaline protease: flavor protease = 2:1:1), enzymatic hydrolysis for 6 hours, centrifuge at 16000r / min, After the clear liquid is filtered through microfiltration-ultrafiltration-nanofiltration membrane, it is spray-dried to obtain clam peptide.

Embodiment 3

[0036] Embodiment 3 of the present invention discloses a preparation method and application of a clam peptide for anti-inflammatory and kidney-protecting, and the adopted technical scheme is as follows:

[0037] Take the whole meat of Hongdao clams, wash and drain, then homogenize, add a certain amount of deionized water to the clam pulp, so that the final clam meat: water = 1:2 weight ratio. Set the enzymatic hydrolysis temperature to 55°C, add 0.2% of the weight of the clam pulp compound protease (the formula is neutral protease: alkaline protease: flavor protease = 2:1:1), enzymatic hydrolysis for 4 hours, centrifuge at 16000r / min, put After the clear liquid is filtered through microfiltration-ultrafiltration-nanofiltration membrane, it is spray-dried to obtain clam peptide.

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Abstract

The invention discloses a preparation method and application of anti-inflammatory and kidney-protecting clam peptide, the preparation method comprises the following steps: taking whole meat of red island clams, washing and crushing to obtain slurry, adding compound protease accounting for 0.1-0.3% of the weight of the slurry, and carrying out enzymolysis, centrifugation, membrane separation and purification and spray drying to obtain clam peptide powder. Red island clams are adopted to prepare the clam peptide, and the clam peptide is used for relieving body inflammatory response and renal function impairment caused by hypertension and is further applied to health-care food with related functions in inflammation and renal injury caused by other diseases.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and more specifically relates to a preparation method and application of a clam peptide for anti-inflammatory and kidney-protecting. Background technique [0002] In real life, the inflammatory response is a moderate or abnormal systemic response of the body's inflammatory immune-related cells according to changes in the internal and external environment. The occurrence of various diseases is related to inflammatory response, so inflammatory response is the basic pathological feature of many diseases, can occur in various tissues and organs, and is also the main link of drug intervention. Inflammation generally triggers changes in the body contents of important inflammatory factors such as IL-6, IL-8, TNF-α, and CRP. At present, common anti-inflammatory drugs mainly include penicillin, cephalosporin, amoxicillin, etc. Long-term use of such drugs will lead to drug resistance in the body and c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/34A61K38/01A61P29/00A61P13/12
CPCC12P21/06C07K1/14C07K1/34A61K38/012A61P29/00A61P13/12C07K14/43504A61K38/00A61P9/12C07K1/36
Inventor 邹圣灿任慧慧刘金丽刘廷廷王尚龙曹廷锋宗建成
Owner CHENLAND NUTRITIONALS INC
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