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Bacillus velezensis D-1 as well as preparation and application thereof

A technology of Bacillus Velez and Bacillus, applied in the field of microbial technology application, can solve problems such as difficulty, long cultivation cycle, physical and mental health, environmental protection and food safety threats, and achieve a significant antagonistic effect

Active Publication Date: 2022-03-01
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology helps protect crops from harmful microorganisms like molds or yeast during harvesting processes. Its use can help reduce damage caused by these organism while still maintain good quality crop products.

Problems solved by technology

This patented technical solution describes how plants have been infected with certain types of harmful or damaging agents that are commonly found on roots called root knotweeds like this one because they attack tubers when stored over time without being consumable themselves. These harmful germs may lead to severe damage if left untreated. To protect against these harms, various techniques were developed including resistant breeding programs, genetic modification of vegetative cells through radiation treatment, chemotherapy, and biocide treatments. However, there has also become apparent concern about potential negative effects from these solutions due to their effectiveness at controlling specific species causing deteriorating quality of the final product.

Method used

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  • Bacillus velezensis D-1 as well as preparation and application thereof
  • Bacillus velezensis D-1 as well as preparation and application thereof
  • Bacillus velezensis D-1 as well as preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] (1) Isolation of Bacillus in soil

[0025] Bacillus was isolated from the tested soil sample (healthy tea tree rhizosphere soil in Wuyi Mountain Tea Seedling Cooperation Base, Nanping City, Fujian Province, China) by dilution coating method. Take 10.0 g of the sterilized and air-dried test soil and put it into a conical flask containing 90 mL of sterile water, mix well, and put the diluted solution in a water bath at 85 °C for 30 min. Dilute to 10 with sterile water -3 、10 -4 、10 -5 、10 -6 Wait for different concentrations of bacterial solution, draw 100 μL of bacterial solution and spread it evenly on the NA plate, place it in a constant temperature incubator at 30°C for 24 hours in the dark, and pick a single colony with obvious differences in colony shape to transfer and purify, and the purified strains are stored in 4 ℃ refrigerator for later use.

[0026] (2) Screening of antagonistic strains and observation of antibacterial morphology against target bacteria ...

Embodiment 2

[0044] (1) Determination of the antagonistic activity of strain D-1 against three kinds of taro dry rot pathogens

[0045] Using the plate confrontation method, the purified Bacillus D-1 was inoculated in NA liquid medium before the experiment, and cultured in a shaker at 28°C and 180rpm for 12h to obtain OD 600 2.0 bio-control bacteria solution. Use a sterile puncher with a diameter of 5 mm to punch out the edge of the pathogenic bacteria and inoculate it in the center of the PDA plate. Spot 1 μL of biocontrol bacteria solution at a distance of 3 cm from the center of the plate, and use 1 μL of sterile water as a control. Repeat 3 times for each treatment . After inoculation, they were cultured in a constant temperature incubator at 30°C for 6 days. Measure control bacterium colony diameter and handle bacterium colony diameter with cross method, calculate its result of antibacterial rate as table 2 and Figure 6 shown.

[0046] Table 2 Antibacterial effect of strain D-1 o...

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Abstract

The invention discloses bacillus velezensis D-1 as well as a preparation and application thereof. The name of the bacillus velezensis D-1 is bacillus velezensis D-1, the bacillus velezensis D-1 is preserved in the China General Microbiological Culture Collection Center on October 12, 2021, the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC NO.23579. The bacillus velezensis D-1 is named as bacillus velezensis D-1. The bacillus velezensis D-1 disclosed by the invention has a remarkable antagonistic effect on three kinds of fusarium causing taro dry rot, also has antagonistic activity on other important plant pathogenic fungi and pathogenic bacteria, and provides a new resource for biological prevention and control of various plant diseases in current agricultural production.

Description

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Claims

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Application Information

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Owner JIANGXI AGRICULTURAL UNIVERSITY
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