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Experimental equipment for placing brain slice

A kind of experimental equipment and brain slice technology, applied in the field of neurophysiological research, can solve the problems of inability to stably obtain high-quality long-term intracellular and extracellular recordings of brain slices, and achieve convenient observation, accurate position, and prevention of uneven height Effect

Pending Publication Date: 2022-02-25
TONGJI UNIV
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  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide an experimental device for placing brain slices, which is used to solve the problem that cannot be stably obtained in the prior art. Problems with high-quality long-term intracellular and extracellular recordings in brain slices

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  • Experimental equipment for placing brain slice
  • Experimental equipment for placing brain slice
  • Experimental equipment for placing brain slice

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Embodiment Construction

[0023] Embodiments of the present invention are described below through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific implementation modes, and various modifications or changes can be made to the details in this specification based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that, in the case of no conflict, the following embodiments and features in the embodiments can be combined with each other.

[0024] It should be noted that the diagrams provided in the following embodiments are only schematically illustrating the basic ideas of the present invention, and only the components related to the present invention are shown in the diagrams rather than the number, shape and shape of the compo...

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Abstract

The invention provides experimental equipment for placing brain slices. The experimental equipment comprises brain grooves, a cover of the brain grooves and a stand column of the brain groove, the brain grooves comprise a first groove, a second groove and a third groove; the first groove is located in the middle of the brain groove, and the first groove is connected with a first perfusion tube; the second groove is connected with the first groove through a second perfusion tube; the third groove is connected with the second groove through a third perfusion tube; the cover of the brain grooves is provided with a gradienter; the number of stand columns of the brain grooves is three, and each stand column is provided with a graduated scale and two rotatable fixing bolts. The experimental equipment for placing the brain slice can ensure that the position of a brain groove is more accurate and stable; moreover, the long-term constant temperature of the perfusate is ensured, and high-quality and long-time internal and external cell records are ensured to be obtained.

Description

technical field [0001] The invention relates to the research field of neurophysiology, in particular to an experimental device for placing brain slices. Background technique [0002] At present, in the field of neurophysiological research, the application of isolated specimens is becoming more and more common, and brain slices are one of them. Not only can evoked field potentials of synapses be recorded on brain slices, but also high-quality intracellular recordings can be obtained. These records can prove that many phenomena on isolated specimens are similar to the overall situation of the original organism where the isolated specimens are located. [0003] In the prior art, when studying isolated brain slices, the brain slices are mainly placed at the bottom of the brain groove, and oxygen-containing artificial cerebrospinal fluid perfusate is poured into the brain slices through an external plastic tube to perform intracellular and extracellular recordings of the brain sl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/00
CPCG01N27/00
Inventor 郑娅杨琦许东升
Owner TONGJI UNIV
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