PSY1 mature polypeptide plant aging inhibitor as well as preparation method and application thereof

A mature polypeptide, plant senescence technology, applied in the directions of botanical equipment and methods, plant growth regulators, plant growth regulators, etc., can solve problems such as no plant senescence, and achieve convenient operation, simple preparation and use methods, and convenient acquisition. Effect

Active Publication Date: 2022-01-11
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are very few studies on the application of mature peptides in plants, especially...

Method used

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  • PSY1 mature polypeptide plant aging inhibitor as well as preparation method and application thereof
  • PSY1 mature polypeptide plant aging inhibitor as well as preparation method and application thereof
  • PSY1 mature polypeptide plant aging inhibitor as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0030] PSY1 mature polypeptide plant senescence inhibitor, including 0.5 μmol / L PSY1 mature polypeptide, 2.8mmol / L 2-(N-morpholine) ethanesulfonic acid solution; The 2-(N-morpholine) ethanesulfonic acid solution is 2 -(N-morpholine)ethanesulfonic acid solution monohydrate was dissolved in MS liquid medium, and the pH of 2-(N-morpholine)ethanesulfonic acid solution was 5.8.

[0031] The preparation method is as follows:

[0032] (1) Weigh PSY1 mature polypeptide powder and dissolve it in water to prepare PSY1 mature polypeptide mother solution with a concentration of 1ɡ / L for later use;

[0033] (2) Weigh quantitative 2-(N-morpholine)ethanesulfonic acid monohydrate, add it to the above-mentioned MS liquid medium, prepare a 2.8mmol / L solution, adjust the pH value of the solution to 5.8, mix and dissolve Uniformly obtain 2-(N-morpholine) ethanesulfonic acid solution;

[0034] (3) Add 38.6 μL of the PSY1 mature polypeptide mother liquor obtained in step (1) to 40 mL of the 2-(N-...

Embodiment 2

[0036] PSY1 mature polypeptide plant senescence inhibitor, including 1 μmol / L PSY1 mature polypeptide, 2.9mmol / L 2-(N-morpholine)ethanesulfonic acid solution; the 2-(N-morpholine)ethanesulfonic acid solution is 2- (N-morpholine)ethanesulfonic acid solution monohydrate was dissolved in MS liquid medium, and the pH of 2-(N-morpholine)ethanesulfonic acid solution was 5.9.

[0037] The preparation method is as follows:

[0038] (1) Weigh PSY1 mature polypeptide powder and dissolve it in water to prepare PSY1 mature polypeptide mother solution with a concentration of 1ɡ / L for later use;

[0039] (2) Weigh quantitative 2-(N-morpholine)ethanesulfonic acid monohydrate, add it to the above MS liquid medium, prepare a 2.5mmol / L solution, adjust the pH value of the solution to 5.9, mix and dissolve Uniformly obtain 2-(N-morpholine) ethanesulfonic acid solution;

[0040] (3) Add 77.2 μL of the PSY1 mature polypeptide mother liquor obtained in step (1) to 40 mL of the 2-(N-morpholine) etha...

Embodiment 3

[0042] PSY1 mature polypeptide plant senescence inhibitor, including 5 μmol / L PSY1 mature polypeptide, 3mmol / L 2-(N-morpholine) ethanesulfonic acid solution; The 2-(N-morpholine) ethanesulfonic acid solution is 2-(N -Morpholine)ethanesulfonic acid solution monohydrate is dissolved in MS liquid medium, and the pH of 2-(N-morpholine)ethanesulfonic acid solution is 5.8.

[0043] The preparation method is as follows:

[0044] (1) Weigh PSY1 mature polypeptide powder and dissolve it in water to prepare PSY1 mature polypeptide mother solution with a concentration of 1ɡ / L for later use;

[0045] (2) Weigh quantitative 2-(N-morpholine)ethanesulfonic acid monohydrate, add it to the above MS liquid medium, prepare a 3mmol / L solution, adjust the pH value of the solution to 5.8, mix and dissolve evenly Obtain 2-(N-morpholine)ethanesulfonic acid solution;

[0046] (3) Take 386 μL of the PSY1 mature polypeptide mother liquor obtained in step (1) and add it to 40 mL of the 2-(N-morpholine)...

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Abstract

The invention provides a PSI1 mature polypeptide plant aging inhibitor and a preparation method and an application thereof, and belongs to the field of plant aging inhibitors, the PSI1 mature polypeptide plant aging inhibitor takes a PSI1 polypeptide mature body as a main functional component, and the PSI1 polypeptide mature body has the following amino acid sequence: D-Y-G-D-P-S-A-N-P-K-H-D-P-G-V-P-P-S. The plant aging inhibitor provided by the invention can inhibit the senescence of leaves of plants, so that the senescence of the plants is delayed, and the plant senescence inhibitor has no other extra bad performance and has extremely strong field operability.

Description

technical field [0001] The invention belongs to the field of plant senescence inhibitors, and in particular relates to a PSY1 mature polypeptide plant senescence inhibitor and its preparation method and application. Background technique [0002] Leaf senescence is an important factor affecting crop yield, quality and other agronomic traits. Taking tobacco as an example, the leaves are the harvesting organs of tobacco. As tobacco enters the late growth period, affected by environmental conditions, the leaves often enter the senescence stage early, reducing the photosynthetic efficiency of the plant and affecting the quality and yield of tobacco. It can effectively delay the aging process of leaves, facilitate the accumulation of assimilated products of leaves, and at the same time, can regulate the consistency of yellowing of tobacco leaves and improve the quality and yield of tobacco. [0003] At present, the application of plant senescence control mainly focuses on the reg...

Claims

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Application Information

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IPC IPC(8): A01N37/46A01N43/50A01N25/30A01N25/02A01P21/00A01G22/45A01G7/06
CPCA01N37/46A01N43/50A01N25/30A01N25/02A01G22/45A01G7/06
Inventor 郭永峰田冬冬邓智超刘涛李伟张增林
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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