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Integrated lossless sampling device for rhizosphere information

A sampling device and rhizosphere technology, applied in the direction of sampling devices, etc., can solve the problems of unseen root exudates, rhizosphere microorganisms and rhizosphere gas, and achieve the effect of reliable and flexible root exudates.

Active Publication Date: 2021-12-17
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no integrated convenient collection device for root exudates, rhizosphere microorganisms and rhizosphere gas

Method used

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  • Integrated lossless sampling device for rhizosphere information
  • Integrated lossless sampling device for rhizosphere information
  • Integrated lossless sampling device for rhizosphere information

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Integrated non-destructive sampling of kidney bean rhizosphere information

[0050] 1. Assembly of Sampling Device

[0051] (1) Stick a 300-mesh spacer on the root growth socket 10, and fill in agar in the through hole;

[0052] (2) The fixed head 12 on the root growth socket 10 is inserted into the baffle plate 13, and glued firmly;

[0053] (3) The inner tube 2 is a silicone tube of 8 × 12mm (referring to the size of the inner diameter and the outer diameter), and 300 mesh nylon mesh 9 is packed into the inner tube;

[0054] (4) observation tube 5 is a silicone tube of 6 × 9mm, one end of which is inserted into the nylon mesh of the inner tube;

[0055] (5) soil crosses the 2mm sieve and packs into observation tube 5 and inner tube 2, the other end of observation tube 5 is connected on the fixed head 12, and the other end of inner tube 2 is sealed with rubber stopper;

[0056] (6) Put an isolation airbag outside the inner tube 2, and then pack into two ho...

Embodiment 2

[0061] Example 2 Integrated non-destructive sampling of rhizosphere information of kidney bean and corn interaction

[0062] 1. Assembly of Sampling Device

[0063] (1) Stick a 300-mesh spacer on the root growth socket 10, and fill in agar in the through hole;

[0064] (2) The fixed head 12 on the root growth socket 10 is inserted into the baffle plate 13, and glued firmly;

[0065] (3) Inner tube 2 is a silicone tube of 13 × 18mm, and 300 mesh nylon mesh 9 is packed into the inner tube;

[0066] (4) The observation tube 5 is a silicone tube of 6×9mm, and the specification of the converging tube is 10×12mm (the joint tube is the same size as this), and then insert one end of the observation tube into the joint tube on the converging tube, and one end of the converging tube Inserted into the nylon mesh of the inner tube;

[0067] (5) soil crosses the 2mm sieve and packs into observation tube 5 and inner tube 2, the other end of observation tube 5 is connected on the fixed he...

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Abstract

The invention relates to the technical field of rhizosphere information acquisition equipment, in particular to an integrated lossless sampling device for rhizosphere information. According to the specific technical scheme, the integrated lossless sampling device comprises a root system independent growth module, the root system independent growth module comprises an outer pipe and an inner pipe which are arranged together in a sleeved mode, the two ends of the inner pipe extend out of the two ends of the outer pipe respectively, and each of the outer pipe and the inner pipe is provided with a plurality of micropores through which a root system cannot penetrate. One end of the inner pipe is sealed through a rubber plug, the other end of the inner pipe is connected with an observation pipe, and the other end of the observation pipe is communicated with a root system growth guide module. The integrated lossless sampling device provided by the invention can be used for carrying out integrated portable collection on root exudates, rhizosphere microorganisms and rhizosphere gas.

Description

technical field [0001] The invention relates to the technical field of rhizosphere information collection equipment, in particular to an integrated non-destructive sampling device for rhizosphere information. Background technique [0002] The rhizosphere is affected by the plant root system, which is different from the non-rhizosphere soil micro-region in terms of physical, chemical and biological characteristics, and is the place where the root system-microbe-soil interface interacts. Studies have shown that the rhizosphere range is about 0.5-4mm. Therefore, for the design of rhizosphere information collection devices, it is first necessary to ensure that rhizosphere samples can be collected accurately, but most current collection devices ignore this point. At the same time, a series of methods and devices for collecting root exudates can collect root exudates of different components, but ignore the interaction between rhizosphere microorganisms, rhizosphere gas and other f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/10G01N1/22
CPCG01N1/10G01N1/22Y02P60/21
Inventor 蒋海波谢芝艺夏映岑阳小成高源桂承波雪峰豪杨称称
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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