Adenosine-participated NMN semi-synthesis method

Abstract

The invention provides an adenosine-participated NMN semi-synthesis method. The adenosine-participated NMN semi-synthesis method comprises the following steps in the same reaction system: (A) carrying out reaction on adenosine, phosphate and saccharides capable of being metabolized by yeast cells under the catalytic action of the yeast cells to generate ATP; and (B) carrying out enzymatic phosphorylation of NR, wherein the corresponding NR and ATP react under the catalytic action of NRK to generate NMN and ADP. In this way, efficient synthesis of the NMN is achieved in the ATP generation and cyclic utilization process, the process is simplified, and meanwhile emission is reduced.

Description

technical field [0001] The invention relates to the technical field of synthesis of beta-nicotinamide mononucleotide (NMN), in particular to a semi-synthetic method of NMN involving adenosine. Background technique [0002] β-nicotinamide mononucleotide (NMN) is the direct precursor of the body to synthesize nicotinamide adenine dinucleotide (NAD). Supplementing NMN is the most effective way to increase the level of NAD in the body. Metabolism has broad and far-reaching health implications. NMN is expected to become a dietary supplement for large-scale applications because the NAD level of the elderly decreases and they cannot obtain enough NMN from food. [0003] At present, the existing synthesis techniques of NMN include four methods: fermentation method, chemical synthesis method, semi-synthesis method and whole enzymatic method. Among them, the fermentation method needs to construct and produce NMN microbial strains, and in the process of mass culture and reproduction ...

AI Technical Summary

Problems solved by technology

There are two main difficulties in the process. One is that a large amount of phosphate accumulated in the reaction will interfere with further reactions. The separation and removal of phosphate is difficult and affects the recovery rate of ATP. The other is that the reaction system involves two enzymes, which require a large amount of enzymes. At the same time, it will inevitably bring many mixed enzymes, and the ...