Full-target antigen-presenting cell tumor vaccine as well as preparation method and application thereof

A technology of tumor vaccines and antigens, applied in the field of biomedical engineering, can solve problems such as low efficiency, and achieve the effects of simple preparation process, good application prospects, and low cost

Pending Publication Date: 2021-09-28
UNIVERSITY OF MACAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the reported nano-vaccine is non-specific uptake by immature DC before antigen present

Method used

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  • Full-target antigen-presenting cell tumor vaccine as well as preparation method and application thereof
  • Full-target antigen-presenting cell tumor vaccine as well as preparation method and application thereof
  • Full-target antigen-presenting cell tumor vaccine as well as preparation method and application thereof

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preparation example Construction

[0034] Some embodiments of the present invention provide a method for preparing an all-target antigen-presenting cell tumor vaccine, which includes: co-incubating quantum dot-modified tumor cells and immature antigen-presenting cells, wherein the quantum dot-modified Tumor cells are obtained by linking quantum dots with protein molecular chains of tumor cells through hydrogen bonds and ligands.

[0035] Specifically, in some embodiments, tumor cells modified with quantum dots are prepared by thermal compounding. The conditions for thermal compounding of quantum dots and tumor cells are as follows: the temperature is 10-100°C, preferably 50-90°C, more preferably 50-80°C, and the compounding time is 1-360min, preferably 1-100min, more preferably 2-30min. For example, heat lamination at 20°C for 200 minutes, or heat lamination at 40°C for 100 minutes, or heat lamination at 80°C for 10 minutes, etc.

[0036] In some embodiments, the thermal compounding process is performed in a s...

Embodiment 1

[0063] This embodiment provides a method for preparing an all-target antigen-presenting cell tumor vaccine, which specifically includes:

[0064] (1) Preparation of carbon quantum dots.

[0065] 1g of citric acid and 3g of urea were dissolved in 30mL of DMSO to obtain a transparent solution, placed in a 50mL polytetrafluoroethylene autoclave, and reacted at 160°C for 4h, and 60mL of ethanol was added to the reacted solution to obtain a black solid, which was washed with water and centrifuged ( 8000rpm, 5min), freeze-dried to obtain dark blue powder, that is, carbon nanodots.

[0066] (2) Thermally compound carbon quantum dots with 4T1 breast cancer cells to obtain quantum dot-modified tumor cells.

[0067] The tumor cell suspension obtained from the shredded tumor tissue obtained from 4T1 breast cancer mice was centrifuged and digested with enzymes supplemented with culture medium; the tumor cell suspension was mixed with inactivated fetal bovine serum, allowed to stand and t...

Embodiment 2

[0071] This embodiment provides a method for preparing an all-target antigen-presenting cell tumor vaccine, which specifically includes:

[0072] (1) Preparation of carbon quantum dots.

[0073] Dissolve 2 g of citric acid and 5 g of urea in 30 mL of DMSO to obtain a transparent solution, place it in a 50 mL polytetrafluoroethylene autoclave, and react for 6 h at 180° C., add 40 mL of ethanol to the reacted solution to obtain a black solid, wash the solid with water, and centrifuge ( 5000rpm, 10min), freeze-dried to obtain dark blue powder, that is, carbon nanodots.

[0074] (2) Thermally compound carbon quantum dots with 4T1 breast cancer cells to obtain quantum dot-modified tumor cells.

[0075] The tumor cell suspension obtained from the shredded tumor tissue obtained from 4T1 breast cancer mice was centrifuged and digested with enzymes supplemented with culture medium; the tumor cell suspension was mixed with inactivated fetal bovine serum, allowed to stand and the cells ...

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Abstract

The invention discloses a full-target antigen-presenting cell tumor vaccine as well as a preparation method and application thereof. The preparation method of the full-target antigen-presenting cell tumor vaccine comprises the following step of co-incubating quantum dot modified tumor cells and immature antigen-presenting cells, wherein the quantum dot modified tumor cells are obtained by connecting quantum dots and protein molecular chains of tumor cells through hydrogen bonds. After the quantum dot modified tumor cells and the immature antigen-presenting cells (APC) are co-incubated, the immature APC (such as MP or DC) can be converted from an immature state to a hyperactivity state, so that the uptake of the tumor antigen is enhanced, the MHC molecular expression is up-regulated on a large scale, and the antigen-presenting efficiency is improved; and meanwhile, the preparation process is simple, the cost is low, and an application prospect is excellent.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to a full-target antigen-presenting cell tumor vaccine and its preparation method and application. Background technique [0002] In recent years, with the development of technology and the improvement of awareness of early cancer screening, the detection rate of various cancers is increasing day by day. However, many tumors are scattered and prone to recurrence, and cannot be completely removed by simultaneous or multiple operations. standard treatment regimen. Existing tumor treatment methods, whether radiotherapy, chemotherapy, or surgical treatment, cannot cure tumor recurrence and metastasis. Antigen-presenting cells (APCs) are known to be indispensable professional cells in the immune system, which can specifically activate T cells and precisely kill tumor cells. After receiving specific activation stimuli, APCs (MP or DC) will migrate to lymphoid organs, and p...

Claims

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Application Information

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IPC IPC(8): A61K39/00A61K47/46A61P35/00C12N5/09
CPCA61K39/0011A61K47/46A61P35/00C12N5/0693A61K2039/5154C12N2502/11C12N2502/1121
Inventor 曲松楠汤子康周胤宁
Owner UNIVERSITY OF MACAU
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