Molecular Markers of DNA Methylation in Large Yellow Croaker and Its Application in Breeding
A technology of molecular markers and large yellow croaker, which is applied in the direction of recombinant DNA technology, DNA/RNA fragments, climate change adaptation, etc., can solve the problems of insufficient molecular regulation mechanism and genetic basis related to growth traits of large yellow croaker, and achieve the promotion of large yellow croaker species. quality, improve quality, and improve the effect of breeding rate
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0063] The method for screening the molecular markers of large yellow croaker DNA methylation of the present invention, comprises the following steps:
[0064] Step 1. The experimental materials come from the "Fufa No. 1" large yellow croaker strain of Ningde Fufa Aquatic Products Co., Ltd., and the large yellow croaker was cultivated in accordance with the technical specifications for large yellow croaker seed breeding of Ningde Fufa Aquatic Products Co., Ltd.; A total of 314 F4 seedlings of the new line about 13 months old were randomly selected from the same net cage as the model. Biological measurements and marks were made for each fish, and the muscles the size of rice grains were cut and stored in absolute ethanol. The group of 40 high-value individuals with the largest body weight was marked as the very large individual group (L group), and the group of 40 low-value individuals with the smallest body weight was marked as the very small individual group (S group).
[006...
Embodiment 2
[0158] The application of molecular markers of large yellow croaker DNA methylation in large yellow croaker breeding includes the following steps:
[0159] Step a, extract DNA from the muscle tissue of large yellow croaker (Shanghai Jierui company DNA extraction kit), after sulfite conversion, use (SEQ ID No.5-SEQ ID No.12) shown in table 1 Primers for PCR amplification;
[0160] Step b, detect PCR amplification product, with reference to the molecular marker fgf11 shown in table 1 (SEQ ID No.1-SEQ ID No.4), molecular marker fgfrs2, molecular marker egflp7 and molecular marker pdgfrα, select fgf11 gene, fgfrs2 Individuals with low average methylation frequency of gene, egflp7 gene and pdgfrα gene were used as breeding parents.
[0161] In summary, the method for screening molecular markers of large yellow croaker DNA methylation provided by the present invention uses WGBS to detect the DNA methylation characteristics of large yellow croaker, and screens the methylation profiles...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com