Markers, primer probes and kit for early screening and diagnosis on endometrial carcinoma

Abstract

The invention discloses markers, primer probes and kit for early screening and diagnosis on an endometrial carcinoma. The markers are partial methylated regions of four genes CDO1, CELF4, HAND2 and HS3ST2, and detection primers and probes are designed for these methylated regions and are all snap-ring-type structures. The kit comprises the primers and the probes. According to the markers, the primer probes and the kit, through screening and combining multiple methylated regions, the most appropriate methylated position is determined for combined diagnosis, and the sensitivity and specificity of detection on early endometrial carcinoma can be remarkably improved. The kit is particularly suitable for carrying out early screening and diagnosis on the endometrial carcinoma by taking a cervical castoff cell as a sample, detection can be achieved in case of a low DNA template concentration, the kit has the advantages of noninvasive sampling, high detection speed, higher sensitivity and specificity, and the like, the accuracy of a result can be ensured so as to assist in achieving the aims of early discovery, early diagnosis and early treatment on the endometrial carcinoma.

Description

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AI Technical Summary

Benefits of technology

This patented technique uses markers on different parts of an organism's body called chromatin regions or nucleic acid sequence targets to determine if they have been affected during menstrual cycles. These areas include both male and female organs such as blood vessels, lymph nodes, nerves, muscles, brain tissue, skin folds, bones, teeth, adhesion points, spines, choroid vascular structures, ovaries, plaque deposits, necrocytes, basement cell masses, spermatozoa, cerebrum squamous epithelium, urinary bladder, lung carcinoma, gastrointestinomas, colon polyps, intrauterine growth retardants, multiplex assays for simultaneously analyzing data obtained at various stages of laboratory examination. Overall, these technical features enhance the accuracy and precision of identifying abnormalities associated with infertiltage caused by esophagchromatic lesions.

Problems solved by technology

This patents discusses various ways to identify abnormal patterns within biological materials such as cell membranes during medical examination procedures like histopathologies. However, current techniques involve analyzing entire genomics databases containing millions of sequences, making interpretation difficult due to lack of comprehensive understanding across different sources. There also exist challenges involving accurate identification of small molecules called chromatin remodeling enzyme targets involved in these processes. Additionally, previous attempts made either require expensive equipment or complex analyzes, leading to delayed treatment options.

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