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A kind of storage method of tussah silkworm egg

A storage method, the technology of tussah eggs, which is applied in the field of crop biological control, can solve the problems that fresh host eggs and bee species cannot be guaranteed to meet in time, extend the shelf life, and parasitic bees are not resistant to cold storage, etc., so as to prolong the effective storage time , reduce losses, and reduce production costs

Active Publication Date: 2022-05-24
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the production process of the flat-bellied wasp, it is impossible to ensure that the fresh host eggs meet the bee species in time. In order to ensure production, it is necessary to preserve the intermediate host tussah eggs
Some parasitic wasps are not resistant to refrigeration, so they can only prolong the shelf life by refrigerating their host eggs.
Due to the different preservation methods, it will directly affect the quality of tussah eggs, the adaptability of the flat-bellied wasp, and the production cost, thereby restricting the large-scale reproduction and utilization of the flat-bellied wasp
[0004] At present, the storage method of tussah eggs is mainly by refrigerating the female moths of tussah moths. This method can only perform laparotomy on the female moths when needed. The operation is cumbersome, and the storage time should not exceed 10 days, which cannot meet the large-scale production of the flat-bellied wasps. need

Method used

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  • A kind of storage method of tussah silkworm egg
  • A kind of storage method of tussah silkworm egg
  • A kind of storage method of tussah silkworm egg

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The storage method of the tussah eggs of the present embodiment, with the tussah eggs with a volume concentration of 0.15% benzalkonium bromide solution sterilization 5min after drying, then 250g / bag, after packing and sealing with a self-sealing bag, carry out the following treatments: A: put Put it in a bucket, press it with a stone, submerge it below the water surface, and place the bucket in a refrigerator at 0-3°C and a relative humidity of 70% for refrigeration.

[0030] In order to illustrate the storage method of the tussah eggs of the embodiment of the present invention, the present embodiment is provided with three groups of comparative samples, and the concrete experimental process and test results are as follows:

[0031] 1 Materials and methods

[0032] 1.1 Materials

[0033] Tested parasitoid: Anastatus fulloi was obtained from lychee stink bug egg mass collected in Litchi Garden of Baiyun Base of Guangdong Academy of Agricultural Sciences. Tussah silkwo...

Embodiment 2

[0055] The storage method of the tussah egg of the present embodiment, the tussah egg is sterilized with a volume concentration of 0.1% benzalkonium bromide solution for 7min and then air-dried, then 250g / bag, after packing and sealing with an aluminum foil bag, carry out the following treatments: A: put Put it in a bucket, press it with a stone, submerge it below the water surface, and place the bucket in a refrigerator at 0-3°C and a relative humidity of 65% for refrigeration.

[0056] The test results of this example show that: after tussah eggs are stored in a water bath at 0-3°C for 180 days, the floating egg rate has no significant difference with CK; the green egg rate has no significant difference with CK; the egg loss rate has no significant difference with CK; The parasitism rate of P. elegans showed a downward trend, but there was no significant difference between 180 days of storage and CK; there was no significant difference between the eclosion rate and CK; compar...

Embodiment 3

[0058] The storage method of the tussah eggs of the present embodiment, with the tussah eggs with a volume concentration of 0.2% benzalkonium bromide solution sterilization after 10min to dry, then 250g / bag, after packing and sealing with a self-sealing bag, carry out the following treatments: A: put Put it in a bucket, press it with a stone, submerge it below the water surface, and place the bucket in a refrigerator at 0-3°C and a relative humidity of 75% for refrigeration.

[0059] The test results of this example show that: after tussah eggs are stored in a water bath at 0-3°C for 180 days, the floating egg rate has no significant difference with CK; the green egg rate has no significant difference with CK; the egg loss rate has no significant difference with CK; The parasitism rate of P. elegans showed a downward trend, but there was no significant difference between 180 days of storage and CK; there was no significant difference between the eclosion rate and CK; compared w...

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Abstract

The invention discloses a method for storing tussah eggs, which comprises the following steps: sealing the tussah eggs, immersing them in a liquid medium, and storing them in cold storage at 0-3°C. The liquid medium may be water. According to the method for storing tussah eggs of the present invention, the tussah eggs are stored in a water bath at 0-3°C for 180 days, and there is no significant difference between the floating egg rate, green egg rate, egg loss rate, eclosion rate, female rate and CK. The storage method of the invention significantly prolongs the effective storage time of the tussah eggs, reduces the loss of the tussah eggs caused by cold storage, and reduces the production cost of the commercial production of the flat-bellied wasp.

Description

technical field [0001] The invention relates to the technical field of crop biological control, in particular to a method for storing tussah eggs. Background technique [0002] Anastatus fulloi belongs to the order Hymenoptera, and is the dominant natural enemy of fruit tree stink bugs. At present, it has achieved large-scale industrial production in southern my country, and it is the main bee that has been successfully used in the field control of lychee stink bugs. kind. At present, this technology has become one of the main biological control methods for litchi bugs in lychee and longan producing areas such as Guangdong, Fujian, Guangxi, Hainan, etc. From 2017 to 2019 alone, the cumulative number of biological control methods in Guangzhou, Huizhou, Maoming, Dongguan, Shenzhen, Zhuhai and Guangxi The demonstration applications in Yulin, Beihai, Hainan Haikou, Sanya, Danzhou, Fujian Zhangzhou, Sichuan Luzhou and other places exceeded 10,000 mu, and the effect of using flat-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02A01K67/04
CPCA01N1/021A01N1/0221A01N1/0215A01K67/04
Inventor 赵灿李敦松张宝鑫冯新霞
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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