A layered liquid pipetting device and method of use thereof

Abstract

The invention discloses a layered liquid pipetting device and a method for using the same. The device includes a pipette and a pipette rack. The upper end of the pipette is provided with a squeeze bag, and the side wall of the lower end of the pipette is provided with a liquid suction port. The pipetting rack includes a pipetting tray, which is provided with a pipette placement hole, and the upper surface of the pipetting tray is provided with a pipetting clip for squeezing the squeeze pack and a pipetting clip for driving the pipetting clip to perform the extrusion action. The liquid driving part, the pipetting tray is connected with the container tray through the lifting part, and the upper end surface of the container tray is provided with a container seat for holding the container and a container driving part for driving the container seat to rotate. Method: Insert the pipette into the pipette placement hole, the pipette driver drives the pipette clip to squeeze the squeeze bag; place the container on the container seat, lower the pipette tray, and make the suction port enter the layered liquid In the layer of liquid to be removed; the pipetting clamp releases the squeeze on the squeeze bag, and the container driver drives the container seat to rotate, so that the liquid suction port absorbs the layer of liquid to be removed in the layered liquid. The invention can improve the liquid pipetting precision.

Description

Technical field [0001] The present invention relates to the technical field of layered liquid separation tools, in particular a layered liquid pipetting device and a method of use thereof. Background [0002] In biological, medical, and chemical experiments, the characteristics of different densities between substances are often used to separate each component by means of density gradient centrifugation. When it is necessary to remove the liquid to be removed in the layer of the layer of liquid in the container, for example, when it is necessary to remove such as Figure 1 When the layer B liquid in the layered liquid in tube 1 shown, pipette 2 needs to be inserted into the layer B liquid for aspirate. [0003] as Figure 2 As shown, the existing pipette 2 is a round tubular structure, the upper end of the pipette 2 is provided with an extrusion package 2-1, the lower end of the pipette 2 is a circular open, this circular open is the pipette 2-2 of the pipette 2. When using pipette...

AI Technical Summary

Problems solved by technology

[0004] like image 3 As shown, because the liquid suction port 2-2 is directly facing the layer C liquid under the layer B liquid, when sucking the layer B liquid, especially when the layer B liquid is relatively shallow, it is very easy to directly place the C layer under the layer B liquid. The layer of liquid is sucked away together, resulting in a reduction in the purity of the pipetted liquid, which also reduces the pipetting accuracy

[0005] Moreover, because when pipetting, the staff needs to hold the test tube 1 with one hand and squeeze or release the squeeze pack 2-1 with the other hand, so the experience and quality requirements of the staff are relatively high, and the staff needs to be highly concentrated Keep your hands steady. Once the staff accidentally inserts the lower end of the pipette 2 into the layer C liquid below the B layer liquid, it is easy to directly suck away the C layer liquid below the B layer liquid. Also reduces pipetting accuracy

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