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Cultivation method for inducing passiflora edulis tetraploid

The invention relates to a technology of Passiflora japonica and a cultivation method, which is applied in the field of inducing tetraploid of Passiflora japonica and can solve the problems of increased processing difficulty, early phenological stage of triploid seedless Lycium barbarum, cumbersome processing process, and the like, To achieve the effect of simplifying the cultivation steps and shortening the cultivation time

Pending Publication Date: 2021-06-29
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are too many seeds in passion fruit juice in the processing process, which increases the difficulty of processing, and the processing process is loaded down with trivial details
The research on seedless fruit has achieved results in some plants, such as the domesticated variety "Guinong No. 49" of seedless thorn pear, which has a large diameter and good taste, which reduces the input in the processing process; triploid seedless watermelon It is easy to eat, good in quality, resistant to disease and waterlogging, high and stable in yield, and resistant to storage and transportation. Strong sex, easy to deep processing; the advantage of seedless fruit is remarkable, but there is no report of seedless purple passionflower in China at present, only Zhang Qin etc. get triploid plant from passionflower endosperm induction

Method used

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  • Cultivation method for inducing passiflora edulis tetraploid
  • Cultivation method for inducing passiflora edulis tetraploid
  • Cultivation method for inducing passiflora edulis tetraploid

Examples

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Effect test

Embodiment 1

[0022] A kind of cultivating method of inducing the tetraploid of Passiflora japonica of the present invention, comprises the steps:

[0023] (1) Use 200mg / L colchicine to carry out mixed culture of the adventitious buds of Passiflora violet. After 15 days of treatment, transfer to the culture medium without adding colchicine for 45 days, and then perform flow cytometry on the adventitious buds. Detection; detection results When the concentration of colchicine is 100mg / L, the induction rate of tetraploid is 22.5%.

[0024] (2) Use 4 mg / L colchicine to soak the adventitious buds of Passiflora japonica for 4 days, then transfer to the culture medium without adding colchicine for 45 days, and then perform flow cytometry on the adventitious buds Detection; the detection result is that the tetraploid induction rate is 17.50% when treated with 3 mg / L colchicine for 4 days. The processing time is 3d.

[0025] (3) Tetraploid leaves induced by colchicine were used as test materials, ...

Embodiment 2

[0028] The difference between embodiment 2 and embodiment 1 is:

[0029] In step (1), utilize the colchicine of 10mg / L to carry out mixed cultivation to the adventitious buds of Passiflora japonica, after processing 15d, change to after continuing to cultivate 45d in the medium that does not add colchicine, to the adventitious buds Carry out flow cytometry; As a result, when the concentration of colchicine is 100mg / L, the induction rate of tetraploid is 17.5%.

[0030] In step (2), utilize the colchicine of 3mg / L to carry out impregnation treatment to the adventitious buds of Passiflora violet, after the treatment time is 3d, after transferring to culture medium without adding colchicine for 45d, the adventitious buds Flow cytometric detection was carried out; the detection result was that the tetraploid induction rate was 17.50% when 3 mg / L colchicine was used for 4 days. The processing time is 5d.

[0031] In step (3), tetraploid leaves induced by colchicine were used as t...

Embodiment 3

[0034] The difference between embodiment 3 and embodiment 1 is:

[0035] In step (1), utilize the colchicine of 0mg / L to carry out mixed cultivation to the adventitious buds of Passiflora violet, after processing for 15d, transfer to the non-added colchicine medium to continue to cultivate after 45d, to the adventitious buds Carry out flow cytometry; As a result, when the concentration of colchicine is 100mg / L, the induction rate of tetraploid is 20%.

[0036] In step (2), utilize the colchicine of 2mg / L to carry out impregnation treatment to the adventitious buds of Passiflora violet, after the treatment time is 5d, after changing to culture medium without adding colchicine for 45d, the adventitious buds Flow cytometric detection was carried out; the detection result was that the tetraploid induction rate was 17.50% when 3 mg / L colchicine was used for 4 days. The processing time is 4d.

[0037] In step (3), tetraploid leaves induced by colchicine were used as test materials...

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Abstract

The invention discloses a cultivation method for inducing a passiflora edulis tetraploid. The cultivation method comprises the following steps: (1) carrying out mixed culture on adventitious buds of passiflora edulis by utilizing colchicine with the concentration of 0-200 mg/L; (2) performing immersion treatment on the adventitious buds of the passiflora edulis by using colchicine with the concentration of 2-4 mg/L; (3) taking tetraploid leaves obtained by colchicine induction as a test material, and averagely differentiating 5-7 adventitious buds from each tetraploid leaf; and (4) carrying out rooting induction by using 1/2MS, 0.5 mg/LIBA and 0.2% of carbon powder, transferring the rooted aseptic seedlings into a normal room for seedling hardening, and transplanting the rooted seedlings. According to the cultivation method, the mutagenic agent is utilized, and the materials are subjected to mixed culture and immersion treatment to obtain variants, so that the culture time is shortened, the culture steps are simplified, and a foundation can be laid for establishing innovation of passiflora edulis seeds.

Description

technical field [0001] The invention relates to the technical field of innovative breeding of nursery stock germplasm, in particular to a cultivation method for inducing tetraploid of Passiflora japonica. Background technique [0002] Passion fruit juice has the reputation of "king of drinks". There are many types of passion fruit juice products on the market, which are widely welcomed by consumers and demand exceeds supply. However, there are too many seeds in the passion fruit juice during processing, which increases the difficulty of processing, and the processing is cumbersome. The research on seedless fruit has achieved results in some plants, such as the domesticated variety "Guinong No. 49" of seedless thorn pear, which has a large diameter and good taste, which reduces the input in the processing process; triploid seedless watermelon It is easy to eat, high in quality, resistant to disease and waterlogging, high and stable in yield, and resistant to storage and tran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H4/00
CPCA01H1/08A01H4/001A01H4/008
Inventor 蔡年辉唐军荣赵李姗朱雅静王雪王亚楠许玉兰
Owner SOUTHWEST FORESTRY UNIVERSITY
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