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Blue-green algae engineering bacterium capable of efficiently fixing nitrogen and preparation method and application thereof

A technology of nitrogen-fixing cyanobacteria and engineering bacteria, applied in the field of industrial microorganisms, can solve the problems of low nitrogen-fixing efficiency of nitrogen-fixing cyanobacteria, and achieve the effect of fast growth rate

Active Publication Date: 2021-06-25
PEKING UNIV
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  • Application Information

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Problems solved by technology

Compared with the amount of fertilizer increase, the nitrogen fixation efficiency of nitrogen-fixing cyanobacteria appears to be relatively low

Method used

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  • Blue-green algae engineering bacterium capable of efficiently fixing nitrogen and preparation method and application thereof
  • Blue-green algae engineering bacterium capable of efficiently fixing nitrogen and preparation method and application thereof
  • Blue-green algae engineering bacterium capable of efficiently fixing nitrogen and preparation method and application thereof

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Embodiment Construction

[0020] The following examples facilitate a better understanding of the present invention, but do not limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent stores. % in the following examples, unless otherwise specified, are mass percentages.

[0021] The pRL277 vector is owned by the laboratory. The vector was donated by Professor Donald A.Bryant, Professor of the Department of Chemistry and Molecular Biology, Pennsylvania State University in 1998. The source of the original literature is T.A.Black, Y.P.Cai and C.P.Wolk, Spatial Expression and Autoregulation of Hetr , a Gene Involved in the Control of Heterocyst Development in Anabaena (Vol 9, Pg 77, 1993), Mol Microbiol 10(5), 1153-1153. Escherichia coli HB101 was purchased from Takara Company, catalog number: D9051....

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Abstract

The invention discloses a cyanobacteria engineering bacterium capable of efficiently fixing nitrogen and a preparation method and application thereof. Anabaena engineering bacterium delta(FTN) of which the heteromorphic cell volume is about two times of the normal heteromorphic cell volume is obtained by knocking out a gene of a cell division related factor FTN of wild anabaena PCC 7120. The nitrogenase activity of the anabaena engineering bacterium delta(FTN) is 2.3 times that of a wild type, the anabaena engineering bacterium delta(FTN) is high in growth speed, the generation time is about 20 hours, the anabaena engineering bacterium delta(FTN) has the prospect of being popularized and applied in agriculture, and a new choice is provided for application of nitrogen-fixing blue-green algae in agricultural production.

Description

technical field [0001] The invention belongs to the field of industrial microorganisms, and in particular relates to a cyanobacteria engineering bacterium with higher nitrogenase activity and its preparation method and application, which provides a new choice for the application of nitrogen-fixing cyanobacteria in agriculture. Background technique [0002] With the development of nitrogen-fixing cyanobacteria in agricultural application basic research, its application potential in agricultural production is getting a more comprehensive and in-depth understanding. Since India first reported using nitrogen-fixing cyanobacteria to fertilize fields in 1939, many studies have proved that rice fields can be inoculated with nitrogen-fixing cyanobacteria to increase production. Stocking nitrogen-fixing cyanobacteria in paddy fields can not only provide nitrogen and organic matter for crops, increase phosphate solubility, reduce water and soil pollution, but also supply oxygen to cro...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N15/74C12N15/65C12N15/31C05F11/08C12R1/01
CPCC05F11/08C07K14/195C12N15/65C12N15/74
Inventor 郑正高王宏蕊董春霞赵进东
Owner PEKING UNIV
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