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Microsatellite marker related to growth traits of takifugu obscurus, primer, method and application thereof

A technique of puffer obscurus and microsatellite marking, which is applied in biochemical equipment and methods, bioinformatics, microbial measurement/inspection, etc., can solve problems such as hindering the progress of pufferfish and improve various growth indicators , high polymorphism, and accurate results

Active Publication Date: 2021-05-14
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]Due to the lack of genome and microsatellite sequences of pufferfish such as pufferfish obscura, many researchers have only been able to design primers using microsatellites of pufferfish obscurus for many years To carry out research related to the screening of functional markers of pufferfish and the evaluation of genetic information of different populations, which has hindered the progress of various researches on pufferfish to a certain extent

Method used

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  • Microsatellite marker related to growth traits of takifugu obscurus, primer, method and application thereof
  • Microsatellite marker related to growth traits of takifugu obscurus, primer, method and application thereof
  • Microsatellite marker related to growth traits of takifugu obscurus, primer, method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0044] Screening of microsatellite markers related to growth traits of Puffer puffer obscura

[0045] (1) Source of material

[0046] Select 300 Fugu obscurus (raised in the same breeding pond) about 5 months old from Jiangyin Shengang Sanxian Breeding Co., Ltd., and measure their total length, body height, body width, head length, eye distance, trunk length, Measurement and recording of 9 growth traits including tail peduncle length, body weight and body length. The normal distribution diagrams of 9 growth traits were established for 300 Fugu obscurus by using SPSS software, and the results were all in line with the normal distribution. Take 16% of high-value individuals (48 individuals), that is, those with a body weight of 42 g or more, and record them as "extremely large groups", and 16% of low-value individuals (48 individuals), that is, those with body weights below 26 g, and record them as "extremely small groups" , 12 individuals were randomly selected from each of t...

Embodiment 2

[0074] Application of Microsatellite Molecular Markers Significantly Correlated with Growth Traits of Puffer Puffer Obscurus in Molecular Breeding

[0075] The 7 microsatellite loci in Example 1 will be used for the verification analysis of the random population in the next step. Using GLM (general linear model) in SPSS to conduct least squares analysis on the correlation between the growth traits of puffer puffer obscura and 7 microsatellite loci, the significance of the difference in growth traits between the same marker genotypes was tested and multiple Compare. The genotypes with less than 3 observations are not statistically analyzed here due to lack of analytical value.

[0076] (1) Association analysis between 7 microsatellite loci and 9 growth traits of Puffer obscurus

[0077] The least squares method was used to analyze the correlation between microsatellite loci and 9 growth traits of puffer puffer, XH11 loci were significantly correlated with body height, body wi...

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Abstract

The invention discloses a microsatellite marker related to growth traits of takifugu obscurus, a primer, a method and an application thereof, the microsatellite markers comprise one or more of XH11, XH47, XH63, XH94 and XH96, and the primers for amplifying the microsatellite markers related to the growth traits of takifugu obscurus comprise five pairs of specific microsatellite primers as shown in SEQ ID NO: 1-10. According to the invention, a microsatellite primer and a plurality of growth traits of takifugu obscurus are subjected to correlation analysis, so that five microsatellite markers (XH11, XH47, XH63, XH94 and XH96) which are remarkably related to the growth traits are screened out. By utilizing the advantages and disadvantage genotypes of different screened microsatellite loci, the breeding of excellent parents of takifugu obscurus can be effectively assisted, the breeding efficiency is improved, and the method is accurate in result, simple and practical.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a microsatellite marker, primer, method and application related to the growth traits of puffer puffer obscura. Background technique [0002] Takifugu fasciatus, commonly known as puffer, belongs to Osteichthyes, Actinopterygii, Letrodontiforms, Tetraodontidae, Takifugu, Domestically, it is mainly distributed in China's Yellow Sea, Bohai Sea and offshore waters, rivers, seas and lakes. Puffer obscurus is a kind of aquaculture fish with high economic, nutritional and breeding value, which is very tender and plump, and has high nutritional value. It is deeply loved by consumers. [0003] The body weight and body length of the wild pufferfish obscura of 3-4 years old were significantly smaller than those of the cultured group. In the breeding process, it is also common that the sexes of the same puffer puffer population have obvious differences in growth traits. Ma...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11G16B20/20
CPCC12Q1/6888C12Q1/6858G16B20/20C12Q2600/156C12Q2600/124C12Q2600/172C12Q2531/113Y02A40/81
Inventor 尹绍武徐杰杰王涛孙亦如储明旭李杰
Owner NANJING NORMAL UNIVERSITY
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