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A kind of cpg-odn with specific immunostimulatory effect on prrsv and application thereof

An immune-stimulating and specific technology, applied in the field of CpG-ODN, can solve problems such as immune-stimulating units that have not yet been seen, and achieve the effects of reducing pig breeding costs, low production costs, and reducing morbidity

Active Publication Date: 2021-06-15
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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Problems solved by technology

There is no research report on the immunostimulatory unit with immunostimulatory effect on PRRSV

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  • A kind of cpg-odn with specific immunostimulatory effect on prrsv and application thereof
  • A kind of cpg-odn with specific immunostimulatory effect on prrsv and application thereof
  • A kind of cpg-odn with specific immunostimulatory effect on prrsv and application thereof

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Embodiment Construction

[0025] The technical solutions of the present invention will be further limited below in conjunction with specific embodiments, but the scope of protection is not limited to the descriptions made. The test methods used in the following examples are conventional methods unless otherwise specified, and the materials and reagents used are reagents or materials that can be directly obtained from commercial channels unless otherwise specified.

[0026] The CpG-ODN fragments are connected end to end to form a DNA sequence containing many -CG-dinucleotides as a CPG motif, which is called "Multi-CpG", that is, the immune activation unit.

[0027] Screening of Example Specific CpG-ODN Fragments

[0028] (1) Screening method: According to conventional techniques, CpG-ODN fragments were directional screened, and the results are shown in Table 1 below.

[0029] Table 1 CpG-ODN fragments screened

[0030]

[0031] The CpG-ODN fragments screened above are screened based on the research...

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Abstract

The present invention relates to the technical field of bioengineering, in particular to a CpG-ODN with a specific immunostimulatory effect on PRRSV and its application. The nucleotide sequence is shown in SEQ ID NO: 1: 11 CpG-ODNs screened out The fragments are randomly composed of 10 groups of immune activation units, and the position information of each CpG-ODN fragment in each group of immune activation units is different. Insert the 10 groups of immune activation units into the multi-copy cloning vector Puc19-derived vector through XbaI and EcoRI restriction sites In FVX, the recombinant plasmid FVX-CpG is constructed by means of gene synthesis, and preparations containing the recombinant plasmid FVX-CpG are prepared. The preparation has low production costs and overcomes the instability, sequence diversity and individual differences of CpG-ODN in vivo. Deficiency: It can not only induce the activation of natural immune function in pigs, but also activate the acquired immune function of PRRSV in pigs, improve the induction of immune responses to PRRSV in pigs, reduce the incidence of PRRSV, and reduce the cost of pig breeding.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a CpG-ODN with specific immunostimulatory effect on PRRSV and its application. Background technique [0002] Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is the causative agent of Porcine Reproductive and Respiratory Syndrome (PRRS). PRRSV was first discovered in the United States in 1987. In 1991, the Dutchman Wensvoot isolated the virus from sick piglets and sows for the first time, and then Germany, the United States, and the United Kingdom also isolated the virus. At present, the virus has spread throughout North America and Europe, and has spread globally, and epidemic infections have occurred successively in various places, causing serious economic losses to the pig industry. Although porcine reproductive and respiratory syndrome virus vaccines have been widely used, they are accompanied by side effects and have only partial immune protection, which m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/117C12N15/85A61K39/39A61P31/14
CPCA61K39/39A61K2039/552A61K2039/55561A61P31/14C12N15/117C12N15/85C12N2310/17C12N2800/107
Inventor 冯璥李涛何文彬吕雪梅
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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