Method for separating and purifying exosomes

Abstract

The invention discloses a method for separating and purifying exosomes. The method comprises the following steps: S1, preparing materials; S2, performing primary separation; S3, performing primary purification; S4, performing filtering and purifying to obtain exosomes with relatively high purity, repeatedly performing filtering twice by using a sterile filter, and filtering impurities to obtain high-purity exosomes; S5, strengthening purification; S6, performing standing and mixing; and S7, collecting the exosomes. By adding agglutinin, a phosphate buffer salt solution, a sucrose solution and an exosome suspension for a cooperative reaction, the purification degree of the exosomes is gradually increased, the operation is simpler, the purification speed is high, filtration is performed through the sterile filter, mixed impurity proteins in the exosomes are effectively removed, the purity and yield of the exosomes are ensured, and a large quantity of polluted proteins in the exosomes are removed through hydroxyapatite and sodium dihydrogen phosphate solutions, so that the purification quality is further guaranteed.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for isolating and purifying exosomes. Background technique [0002] Exosomes are 30-200nm-diameter encapsulated cystic vesicles with double-layered lipid molecules, which are selectively packaged and released by living cells, and are abundant in human body fluids. Exosomes contain different types of Lipids, nucleic acids, and proteins, etc., related studies have also shown that exosomes play a huge role in intercellular communication and physiological and pathological processes; at present, the removal of foreign proteins in exosomes is an urgent solution The problem is that in the existing separation and purification methods, the exosome suspension still contains a large amount of impurity proteins, which are not pure exosomes, and the purification degree is low and the speed is slow. Contents of the invention [0003] The purpose of the present invention is ...

AI Technical Summary

Problems solved by technology

[0002] Exosomes are 30-200nm-diameter encapsulated cystic vesicles with double-layered lipid molecules, which are selectively packaged and released by living cells, and are abundant in human body fluids. Exosomes contain different types of Lipids, nucleic acids, and proteins, etc., related studies have also shown that exosomes play a huge role in intercellular communication and physiological and pathological processes; at present, the removal of foreign proteins in exosomes is an urgent solution However, in the existing separation and purification methods, the exosome suspension still contains a large amount of impurity proteins, which are not pure exosomes, and the purification degree is low and the rate is slow.