Culture media for culturing wild kentucky bluegrass

A technology of bluegrass and rooting medium is applied in the directions of application, plant regeneration, horticulture, etc., can solve problems such as difficulty in continuation of medium effect, and achieve the effects of reducing seedling cultivation cost, good application prospect and shortening cultivation period.

Active Publication Date: 2021-02-26
内蒙古蒙草草种业有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tissue culture propagation of wild bluegrass generally includes three stages: primary induction→subculture multiplication→rooting culture. It is relatively easy to find the medium for each individual link, but it is difficult to achieve the continuation of the effect of the medium at each stage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A culture medium for wild bluegrass cultivation, comprising the following steps:

[0052] Step 1: the explants are taken from the growing points on the rhizomes of wild bluegrass; the explants are sterilized for later use;

[0053] Step 2: Inoculate the explants on the first-generation induction medium, and perform the first-generation induction culture to differentiate clustered buds;

[0054] The primary induction medium is:

[0055]1 / 2MS+6-BA0.5mg / L+NAA 0.1mg / L+B 9 1mg / g;

[0056] The conditions for the first-generation induction culture are culture temperature 23±1°C, light intensity 1500Lx, no supplemental light at night except natural light during the day, and a culture period of 40 days;

[0057] Step 3: Cultivate the clustered shoots of the primary induction culture on the subculture medium; transfer the better seedlings in the subculture medium to the rooting medium to induce rooting;

[0058] The subculture medium is:

[0059] Improved MS+ 6-BA 0.5mg / L+K...

Embodiment 2

[0071] A culture medium for wild bluegrass cultivation, comprising the following steps:

[0072] The primary induction medium is:

[0073] 1 / 2MS+6-BA0.4mg / L+NAA 0.1mg / L+B 9 0.8mg / g;

[0074] The subculture medium is:

[0075] Improved MS+ 6-BA 0.5mg / L+KT 0.5mg / L+NAA 0.1mg / L+B 9 1.0mg / L

[0076] The improved MS formula is: the content of ammonium nitrate and potassium nitrate in macroelements is reduced by 45%, the content of magnesium sulfate heptahydrate is increased by 15%, and the rest of the ingredients remain unchanged;

[0077] Described rooting medium is:

[0078] 1 / 2MS+B 9 0.95mg / L+NAA 0.45mg / L+AC 1.0mg / L.

[0079] All the other steps are the same as in Example 1.

Embodiment 3

[0081] A culture medium for wild bluegrass cultivation, comprising the following steps:

[0082] The primary induction medium is:

[0083] 1 / 2MS+6-BA0.6mg / L+NAA 0.1mg / L+B 9 1.2 mg / g;

[0084] The subculture medium is:

[0085] Improved MS+ 6-BA 0.5mg / L+KT 0.5mg / L+NAA 0.1mg / L+B 9 1.0mg / L

[0086] The improved MS formula is: the content of ammonium nitrate and potassium nitrate in macroelements is reduced by 55%, the content of magnesium sulfate heptahydrate is increased by 18%, and the rest of the ingredients remain unchanged;

[0087] Described rooting medium is:

[0088] 1 / 2MS+B 9 1.05mg / L+NAA 0.55mg / L+AC 1.0mg / L.

[0089] All the other steps are the same as in Example 1.

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PUM

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Abstract

The invention discloses culture media for culturing wild kentucky bluegrass. The culture media comprise a primary induction culture medium, a subculture medium, a rooting culture medium and a seedbedculture medium; the primary induction culture medium is composed of 1 / 2MS, 0.4-0.6 mg / L of 6-BA, 0.1 mg / L of NAA and 0.8-1.2 mg / g of B9; and the subculture medium is composed of an improved MS, 0.5 mg / L of 6-BA, 0.5 mg / L of KT, 0.1 mg / L of NAA and 1.0 mg / L of B9, wherein the formula of the improved MS is characterized in that the content of ammonium nitrate and potassium nitrate in macroelements is reduced by 45%-55%, the content of magnesium sulfate heptahydrate is increased by 15%-18%, and the rest components are not changed. According to the culture media, the culture period is shortened bynearly one third, the seedling pollution rate is reduced to 5% or below, and the survival rate reaches 95% or above; and the seedling cultivation cost is reduced by more than 20%.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, and in particular relates to a culture medium for wild bluegrass cultivation. Background technique [0002] Wild bluegrass, alias, is in great demand. [0003] Plant tissue culture is a very important means of biotechnology. Plant cells, stem segments, leaves, etc. can be cultured. The cells, organs and tissues of plants are taken and put into containers with appropriate culture medium, and can be differentiated to form complete plants under certain culture conditions. As a powerful means of biotechnology, tissue culture has been paid more and more attention. Tissue culture Under in vitro culture conditions, different plants and tissue cells in different parts of the same plant have different nutritional requirements. Only when their special requirements are met can they grow and develop better. Mastering the preparation and screening methods of medium is one of the key links to achieve the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 马怀林贾振宇高俊刚张跃华高旭刘亚玲刘思泱屈璐璐王乌恩巴图
Owner 内蒙古蒙草草种业有限公司
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