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Method for promoting propagation of bait microalgae

A technology for feeding microalgae and Nannochloropsis, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of not considering the influence of interalgal bacteria, etc., and is beneficial to the control of the amount of microalgae and the production management. Effect

Active Publication Date: 2021-02-12
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the actual microalgae propagation technology does not consider the influence of algae bacteria.

Method used

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  • Method for promoting propagation of bait microalgae
  • Method for promoting propagation of bait microalgae
  • Method for promoting propagation of bait microalgae

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Example 1: Screening Beneficial Bacteria

[0018] The aseptically purified Thalassiosira, Nannochloropsis, and phycobacteria isolated from the algae liquid were cultured to the exponential growth phase, respectively. Thalassiosira micronana and Nannochloropsis algae liquid were divided into 100mL sterile Erlenmeyer flasks by 50mL each. Each expanded bacteria solution was collected by centrifugation at 13000 rpm at low temperature for 10 minutes and washed three times with Ningda No. 3 seawater medium to remove the 2216E liquid medium. According to the ratio of algal cell density ratio of 1:50, different algae bacteria were added to the Thalassiosira micronana and Nannochloropsis culture medium respectively, and each group was set up in three parallels, and Ningda No. 3 seawater culture medium was added as a control Group. Taking the cell density of Thalassiosira micronana and Nannochloropsis as the index, the effect of various phycobacteria on the growth of Thalassios...

Embodiment 2

[0021] Embodiment 2: Propagation of bait microalgae

[0022] First prepare algae expansion culture solution, its composition is as follows: 50-200mg / L KNO 3 , 5-20 mg / L KH 2 PO1-5 mg / LFe-citrate·5H 2 O, 0.01-0.1g / L brown sugar, 2-10μg / L VB 1 , 0.01-0.1μg / L VB 12 .

[0023] The concrete composition of a kind of algae expansion culture liquid is as follows:

[0024] 100mg / L KNO 3 , 10mg / L KH 2 PO 4 , 3mg / L Fe-citrate 5H 2 O, 0.6 g / L brown sugar, 6 μg / LVB 1 , 0.05μg / L VB 12 .

[0025] Brown sugar can be selected from the varieties commonly used in the market.

[0026] A kind of specific preparation method of algae expansion culture liquid is as follows:

[0027] (1) Prepare 50mL filtered seawater (salinity 25‰), add 5mg KNO 3 , 0.5mg KH 2 PO 4 , 0.15mg Fe-citrate·5H 2 O for use after sterilization.

[0028] (2) Dissolve 3 g of brown sugar in 10 mL of pure water, pass through a 0.22 μm filter membrane, and add 10 μL to the above 50 mL of the sterilized solution. ...

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Abstract

The invention discloses a method for promoting propagation of bait microalgae. The invention provides a method for expanding propagation of bait microalgae. The highest propagation density of the baitmicroalgae is increased and the platform period maintaining time of the bait microalgae is prolonged by adding an algal beneficial bacterium separated from a pseudominiature sea chain algae solutionand a nannochloropsis oculata algae solution. The phycomycetes beneficial bacteria are bacillus marinus with the preservation number of CCTCC M 20200448. According to the method disclosed by the invention, the propagation speed of the nannochloropsis oculata and the nannochloropsis oculata is much higher than the microalgae culture speed of a conventional microalgae nutrient solution, the highestpropagation density and the plateau period maintenance time are much higher than those of a conventional culture method, more microalgae can be supplied under the same water body condition, and meanwhile, the plateau period is relatively often not easy to decay, so the microalgae quantity control and the production management in the actual shellfish large-scale production process are very facilitated.

Description

technical field [0001] The invention belongs to the technical field of aquaculture bait microalgae cultivation, and specifically relates to a method for promoting the proliferation of bait microalgae, which can effectively improve the maximum breeding density of important bait microalgae such as Thalassiosira pseudonana and Nannochloropsis and maintain the plateau period time. Background technique [0002] Scholars at home and abroad have found in the process of shellfish cultivation that there is currently no artificial bait that can be used for seedling breeding of tidal flat shellfish. Even if microalgae dry powder or refrigerated microalgae are used, the effect is far inferior to that of live microalgae. (Espinosa et al., 2006), so in the actual production of shellfish fry, only freshly cultured living microalgae cells are the only bait choice for shellfish fry cultivation. Among them, the microalgae with better bait effect mainly include Isoflagellates, Thalassiosira p...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/20C12N1/38C12R1/89C12R1/01
CPCC12N1/12C12N1/20C12N1/38
Inventor 曹嘉懿凌婷徐继林周成旭严小军
Owner NINGBO UNIV
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