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Molecular marker for gene-assisted breeding related to rape dominant cell nucleus sterility and application of molecular marker

A molecular marker and assisted breeding technology, which can be used in application, plant genetic improvement, and microbial assay/inspection. , to achieve the effect of single plant identification and selection of recurrent selection population, simple identification method, and expansion of germplasm base

Active Publication Date: 2021-01-22
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also obvious shortcomings in recessive genic male sterility: it is difficult to obtain all sterile populations, and 50% of the fertile plants in the sterile line must be manually removed during reproduction and seed production
Therefore, how to remove 50% of the fertile lines in the female parent line in an economical and effective way is the main problem to be solved in the wide application of nuclear sterility; at the same time, it is more difficult to transfer the 8029AB nuclear male sterile two-type male sterile line

Method used

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  • Molecular marker for gene-assisted breeding related to rape dominant cell nucleus sterility and application of molecular marker
  • Molecular marker for gene-assisted breeding related to rape dominant cell nucleus sterility and application of molecular marker
  • Molecular marker for gene-assisted breeding related to rape dominant cell nucleus sterility and application of molecular marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Genetic Analysis of 8029AB Rapeseed Nuclear Dominant Sterile Material

[0074] 1. Experimental materials

[0075] The material used in this experiment is rapeseed nuclear dominant three-line 8029AB, in which the sterile line is called 8029A, the fertile line is called 8029B, and the restorer line is 6449. The above materials are all from the Institute of Oil Crops, Chinese Academy of Agricultural Sciences.

[0076] Rapeseed rape nuclear dominant three-type line 8029AB Material source: 3 sterile individual plants were obtained from the rapeseed inbred line 8029, and the sterile individual plants were crossed with the rapeseed inbred line Zhongshuang 11, and the F1 of the first generation was crossed with Zhongshuang 11 No. was backcrossed as a recurrent parent to obtain a near-isogenic line.

[0077] 2. Genetic law of nuclear dominant sterile material

[0078] Using classical genetics methods, the F1 and backcross segregation population obtained by crossing sterile mat...

Embodiment 2

[0082] Genetic Analysis of 8029A Restorer Line of Rapeseed Nuclear Dominant Sterile Material

[0083] F1, F2 and backcross segregation populations obtained by crossing the sterile material 8029A as the female parent with the restorer line 6449 were analyzed. The test results showed (Table 2): the restorer line of the sterile material 8029A was controlled by a pair of nuclear genes, and closely linked with the sterile gene.

[0084] Table 2 Genetic analysis of the 8029A restorer line of dominant nuclear male sterile material

[0085]

Embodiment 3

[0087] Acquisition of Molecular Markers in Rapeseed Rapeseed Nuclear Dominant Three-line 8029AB Assisted Breeding

[0088] 1. Primer design according to BnMS5 a (KX223882), BnMS5 c (KX223881) and BnMS5 e (SEQ ID NO: 7) gene sequence and the SNP sites on both sides nearby for mining. The selected SNP loci were selected, and primers were designed using primer design software, and two primers were designed for each set of markers. The primers were synthesized by Qingke Company.

[0089] 2. PCR reaction detection

[0090] In Example 1 and Example 2, the DNA of part of the sterile and fertile strains of the BC1 segregation population was used as a template, and the primer SNPMS5 a -5F / R, SNPMS5 c -3F / R, SNPMS5 e -1F / R was subjected to PCR amplification.

[0091]The reaction system of PCR is: Taq DNA Polymerase 5U, 10×TaqBuffer 2.4μL, 2.5mM dNTPMixture 0.8μL, 10uM forward and reverse primers 0.2μL each, 2.5U / μL DNA Polymerase 0.2μL, template DNA 50ng, ddH 2 0 to 15 μL;

[...

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Abstract

The invention discloses a molecular marker for gene-assisted breeding related to rape dominant cell nucleus sterility and application of the molecular marker, and relates to the technical field of rape molecular breeding. The invention provides the molecular marker for gene-assisted breeding related to rape dominant cell nucleus sterility, an identification primer, a kit and application in dominant cell nucleus male sterility heterosis utilization assisted breeding of rape and related crops. The molecular marker has the advantages of simplicity in operation, low cost, short period and the like; and moreover, the marker has high stability, is not influenced by environmental factors, and can be used for performing molecular marker assisted selection in early generations, shortening the breeding period and improving the breeding efficiency. The dominant cell nucleus male sterility molecular marker assisted selection breeding of the rape and related crops can be realized by utilizing molecular marker gene sequence information; dominant cell nucleus three-line hybrid seed production of the rape and related crops can be realized; and single plant identification selection of recurrent selection populations of the rape and related crops can be realized.

Description

technical field [0001] The invention belongs to the technical field of molecular breeding of rapeseed, and in particular relates to a molecular marker of gene-assisted breeding related to dominant cell sterility of rapeseed and its application. Background technique [0002] Male sterility is the most economical and effective way to utilize crop heterosis. At present, in the utilization of heterosis in rapeseed internationally, the main types of male sterility used are: MSL (Europe and North America), PGS (North America), OGURA-INRA (North America and Europe), Polyma CMS (China). Both MSL and PGS belong to nuclear male sterility, the hybrids of the MSL system account for 70-80% of the European hybrids, and the hybrids of the PGS system account for 40% of the Canadian market (2006). Nuclear male sterility has many advantages in the utilization of heterosis: the sterility is complete and stable, and only two lines are needed for matching, which greatly shortens the breeding cy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11A01H1/02A01H1/04
CPCC12Q1/6895A01H1/02A01H1/04C12Q2600/172C12Q2600/156C12Q2600/13
Inventor 曾新华闫晓红吴刚袁荣王淼罗军玲刘芳朱莉李晓飞李均
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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