Cell exosome purification device and method

Abstract

The invention relates to a cell exosome purification device and method. The purification device comprises a first hollow fiber column and a second hollow fiber column; the two ends of an inner cavityof the first hollow fiber column communicate with a stock solution bottle to form a refined filtration loop; the two ends of an inner cavity of the second hollow fiber column communicate with a transfer bottle to form a concentration loop; an outer cavity of the first hollow fiber column is connected with the transfer bottle through a filtrate pipeline, and the filtrate pipeline is connected witha first manual clamp; a first filter membrane and a first peristaltic pump are sequentially connected to a liquid outlet of the stock solution bottle on the refined filtration loop; and a second peristaltic pump is connected to a liquid outlet of the transfer bottle on the concentration loop. According to the purification device disclosed by the invention, the exosome cell stock solution is filtered by the first hollow fiber column and then concentrated by the second hollow fiber column, so that the purification device is suitable for large-scale continuous automatic sample loading and concentration of a large amount of supernatant, the manual operation time is greatly saved, and the exosome-containing supernatant can be prepared on a large scale; and standardization of exosome preparationis realized.

Description

technical field [0001] The invention relates to the technical field of cell culture and purification, in particular to a cell exosome purification device and method. Background technique [0002] Exosomes are phospholipid bilayer-encapsulated vesicles released into the extracellular environment after the fusion of intracellular multivesicular bodies and the cytoplasmic membrane, with a diameter of about 40-100 nm, and their abundance in serum and body fluids is high. But the yield of exosomes isolated from blood or body fluids is very low. [0003] Isolation of exosomes from blood or cell supernatant is a complex project. Existing exosome isolation techniques, such as centrifugation, ultracentrifugation, magnetic bead separation, and affinity-capture-based methods, are time-consuming and suffer from poor purity, throughput, and reproducibility. Among them, centrifugation and ultracentrifugation are time-consuming and low in purity. Magnetic beads and affinity capture metho...

AI Technical Summary

Problems solved by technology

Existing exosome isolation techniques, such as centrifugation, ultracentrifugation, magnetic bead separation, and affinity capture-based methods, are time-consuming and suffer from poor purity, throughput, and reproducibility

Among them, centrifugation and ultracentrifugation are time-consuming and low in purity. Magnetic beads and affinity capture methods are likely to cause surface modification of exosomes, which affect the characteristics of exosomes and are not easy to remove, which is not conducive to subsequent functional analysis.

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