Complex and detection device

A detection device and composite technology, applied in the field of composites, can solve the problems of easy fading, weak π bonds in chemical structure, etc., and achieve the effect of stable luminescence characteristics and good dispersion

Pending Publication Date: 2021-01-05
PANASONIC INTELLECTUAL PROPERTY MANAGEMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the fluorescence method, in order to label the target substance, an organic fluorescent dye is used, but since the organic fluorescent dye has a chemically weak π bond, it is easy to fade

Method used

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  • Complex and detection device
  • Complex and detection device
  • Complex and detection device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0081] Hereinafter, the complex according to Embodiment 1 and a detection device using the complex will be described.

[0082] [Complex]

[0083] First, refer to Figure 1 ~ Figure 3 The complex according to Embodiment 1 will be specifically described. figure 1 It is a schematic configuration diagram showing an example of the configuration of the complex 2032 according to the first embodiment.

[0084] Such as figure 1 As shown, the complex 2032 includes a first substance 20322, a quantum dot 20321a, and a linking substance 20321b. The first substance 20322 has a property of being able to specifically bind to a target substance to be detected. Examples of target substances include proteins, lipids, sugars, and nucleic acids. Substances capable of specifically binding to a target substance include, for example, antibodies against antigens, enzymes against substrates or coenzymes, receptors for hormones, protein A or protein G against antibodies, and antibiotics against bio...

Embodiment 1～3

[0106] In the following examples, the compound (APDMES) represented by the above structural formula (2) was used as a linking substance. As shown in the above structural formula (2), APDMES has an amino group (-NH 2 base), and 1 silanol group. In addition, as quantum dots, silicon microparticles with a particle diameter of 3.9 nm doped with phosphorus and boron were used. In addition, DMT-MM was used as a substance that promotes the reaction between quantum dots and linking substances.

[0107] The surface-modified quantum dots involved in Examples 1 to 3 were mixed in such a way that the mixing ratio of the quantum dots and the linking substance became 1:125, 1:250, and 1:1250 (the number of quantum dots: the number of molecules of the linking substance), according to Figure 4 Fabrication method shown. Next, the surface-modified quantum dots in Examples 1 to 3 were respectively dispersed in water to prepare a dispersion (hereinafter referred to as an aqueous dispersion o...

Embodiment 4

[0120] according to Figure 5 In the production method shown, a complex in which the surface-modified quantum dots involved in Example 2 were bound to an IgG antibody was produced. The obtained complex was dispersed in phosphate-buffered saline (PBS: Phosphate Buffered Salts) to prepare a dispersion. The light transmittance of this dispersion liquid was measured by the same method as Examples 1-3. show the result in Figure 8 middle. Figure 8 It is a graph showing the measurement results of the light transmittance of the dispersion liquid of the complex according to Example 4. In addition, Reference Example 2 is a dispersion liquid in which the surface-modified quantum dots according to Example 2 are dispersed in PBS, and Reference Example 3 is a dispersion liquid in which IgG is dispersed in PBS.

[0121] Such as Figure 8 As shown, the light transmittance of the dispersion liquid of the complex according to Example 4 is almost equal to the light transmittance of the di...

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Abstract

The present invention provides a complex having excellent dispersibility and stable light-emitting properties. The complex (2032) contains a first substance (20322) having the property of binding specifically to a target substance, a quantum dot (20321a) containing silicon as the main component and having a negatively charged surface, and a linker substance (20321b) containing the compound represented by general formula (1) and covering the surface of the quantum dot (20321a), the first substance (20322) being fixed onto the surface of the quantum dot (20321a) via the linker substance (20321b). (1) X-L-Si-(R1)(R2)(OR3) (In the formula, X represents a basic functional group, R1, R2, and R3 each independently represent an alkyl group, and L represents an alkylene group).

Description

technical field [0001] The present disclosure relates to a complex that specifically binds to a target substance and a detection device using the complex. Background technique [0002] Fluorescence methods such as surface plasmon resonance fluorescence analysis (surface plasmon-field enhanced fluorescence spectroscopy: Surface Plasmon-field enhanced Fluorescence Spectroscopy) are known as one of detection methods for detecting a trace amount of target substances with high sensitivity. In the fluorescence method, an organic fluorescent dye is used to label a target substance, but since the organic fluorescent dye has a chemically weak π bond, it tends to fade. Therefore, there is a demand for fluorescent substances that do not fade and have stable luminescent properties. [0003] As a fluorescent substance having stable light-emitting properties, for example, a light-emitting material composed of an inorganic substance can be considered. For example, Patent Document 1 discl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543B82Y15/00C01B33/02C09K11/59G01N21/41G01N21/64
CPCB82Y30/00C09K11/025C09K11/59C01B33/02G01N33/543B82Y15/00B82Y40/00C09K11/02G01N21/6428G01N21/648G01N2021/1727G01N2021/258
Inventor 柳川博人
Owner PANASONIC INTELLECTUAL PROPERTY MANAGEMENT CO LTD
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