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High-sensitivity rapid immunodetection method based on acoustic microfluid and application

A rapid immunoassay and high sensitivity technology, applied in the field of biomedical immunoassay, which can solve the problems of limited sensitivity and rapid increase in speed

Pending Publication Date: 2020-10-30
BEIJING UNIV OF CHEM TECH +1
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

This greatly limits the rapid improvement of sensitivity and speed

Method used

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  • High-sensitivity rapid immunodetection method based on acoustic microfluid and application
  • High-sensitivity rapid immunodetection method based on acoustic microfluid and application
  • High-sensitivity rapid immunodetection method based on acoustic microfluid and application

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Embodiment 1

[0028] Such as Figure 4 Shown is a typical immunoassay process provided in this example, the chemiluminescence method is used to detect interleukin-6, and the specific steps include:

[0029] 1. Surface activation of acoustic microfluidic devices: Oxygen plasma was applied for 5 min cleaning and hydrophobic treatment, then, incubation was carried out in 20 mM 3-aminopropyltrimethyloxysilane for 10 h, and finally, with toluene and methanol cleaning.

[0030] 2. Use a syringe pump to coat and block the surface of the device with the capture antibody. The capture antibody is an interleukin-6 antibody, and the solution used for surface blocking is a PBS solution with a concentration of 1% BSA.

[0031] 3. Add the sample to be tested and turn on the bulk acoustic wave device at the same time to realize the enrichment of the sample to be tested in the designated area. After the reaction is completed, turn off the bulk acoustic wave device and add cleaning solution (10mM sodium pho...

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Abstract

The invention relates to a high-sensitivity rapid immunodetection method based on acoustic microfluid and application. The immunodetection method comprises the steps that the acoustic microfluid is adopted for enriching effective substances in a reagent, and then acoustic waves are utilized for cleaning, wherein the cleaning is to desorb non-specifically adsorbed molecules by using acoustic microfluid. According to the immunodetection method provided by the invention, molecular enrichment and non-specific desorption are combined based on acoustic microfluid, so that the immunodetection sensitivity is improved, the detection speed is increased, high-sensitivity and rapid immunodetection is realized, and the immunodetection method has a wide market prospect.

Description

technical field [0001] The invention relates to the technical field of biomedical immunoassay, in particular to a high-sensitivity and rapid immunoassay method and application based on acoustic wave microfluidics. Background technique [0002] Microfluidic technology has the advantages of less reagent consumption, low cost, and high integration. It has shown good application prospects in the field of environmental monitoring, and can meet the development direction of portable and miniaturized analytical instruments. It is especially important for the rapid detection of immunoglobulin and the early diagnosis of infectious diseases. Immunological detection is an important means of diagnosing infectious diseases. In the past few decades, this field has developed rapidly, and scientists have invented a variety of immunoassay methods. Due to the low concentration of antigen / antibody in the positive serum of patients at the early stage of onset, it has higher requirements for de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53B01L3/00
CPCG01N33/53B01L3/502761B01L3/50273B01L2400/0436
Inventor 郭霄亮陈岩
Owner BEIJING UNIV OF CHEM TECH
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