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A kind of micro-ecological composite additive containing Saccharomyces cerevisiae and its application

A compound additive, the technology of Saccharomyces cerevisiae, which is applied to Saccharomyces cerevisiae and its application in aquaculture, can solve the problems of normal flora structure destruction, pathogenic bacteria resistance, endangering human health and other problems, so as to reduce the probability of disease occurrence, The effect of improving meat quality and promoting animal growth

Active Publication Date: 2021-05-28
青岛玛斯特生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive use of antibiotics will not only destroy the normal flora structure of the prawn intestinal tract, but also easily lead to drug resistance of pathogenic bacteria. Drug-resistant strains enter the human body through the water body or prawns, and eventually endanger human health. These phenomena have attracted people's attention. of high concern

Method used

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  • A kind of micro-ecological composite additive containing Saccharomyces cerevisiae and its application
  • A kind of micro-ecological composite additive containing Saccharomyces cerevisiae and its application
  • A kind of micro-ecological composite additive containing Saccharomyces cerevisiae and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Isolation, screening and identification of strains

[0029] 1. Sample:

[0030] It was collected from a Litopenaeus vannamei farm in Rizhao City. Healthy and vigorous prawn intestines were picked by aseptic operation for subsequent separation.

[0031] 2. Screening method

[0032] Take the intestinal sample and put it into a grinder, put it into a normal saline, homogenize it, and centrifuge at a low speed, so that the bacteria in the sample are fully dispersed in the upper layer of normal saline, dilute it in a 10-fold gradient, and inoculate it on 2216E, TSB and MRS solid medium respectively. Cultivate at 28°C for 24h-48h. Select a uniform and clear single colony and streak to purify the bacteria. The single colonies were named MST01, MST02, MST03..., MST30 accordingly.

[0033] Using Vibrio parahaemolyticus and Vibrio harveyi as indicator bacteria, probiotics were screened by plate antagonism method. Spot the bacterial liquid to be screened on a pl...

Embodiment 2

[0042] Example 2 Effects of Saccharomyces cerevisiae MST01 on growth, immunity and stress resistance of Litopenaeus vannamei

[0043] In this experiment, a control group and a probiotic group were set up, and each group was set up in three parallels, with 200 Litopenaeus vannamei in each parallel. The high-temperature-cured commercial compound feed is used as the basic feed. Among them, the control group was fed with basal feed, and the probiotic group was fed with 10 7 CFU / g feed for S. cerevisiae MST01. The breeding experiment lasted for 56 days. The prawns were fed at 3-5% of their body weight every day, and the amount of bait was adjusted at any time according to the food intake. They were fed once a day in the morning and evening, and sucked and discharged once a day. During the experiment, the dissolved oxygen was ≥7mg / l, the temperature was 29±1°C, the salinity was 21-22‰, and the pH was 8.0±0.3. After the breeding experiment, the Litopenaeus vannamei were counted an...

Embodiment 3

[0052] Example 3 Effects of Saccharomyces cerevisiae MST01 on muscle quality and fatty acid composition of Litopenaeus vannamei

[0053] Saccharomyces cerevisiae MST01 was added to the diet of Litopenaeus vannamei, and the texture analyzer and gas chromatography were used to analyze the changes of muscle texture and fatty acid content of the shrimp after feeding, so as to study the effect of Saccharomyces cerevisiae MST01 on the muscle quality of Litopenaeus vannamei.

[0054] 1. Preparation of bacterial suspension

[0055] Saccharomyces cerevisiae MST01 was inoculated in the nutrient medium with an inoculum of 1%, cultured statically at 37°C for 20 hours, centrifuged at 4000r / min for 15 minutes after cultivation, washed 3 times with sterile normal saline, and resuspended in sterile normal saline , adjust the bacterial suspension concentration to 10 9 CFU / mL.

[0056] 2. Preparation of bacteria-containing feed

[0057] Add the treated bacterial suspension to the commercial ...

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Abstract

The invention relates to the technical field of functional microorganism screening and application, and specifically provides a microecological composite additive and its application in aquaculture. Described micro-ecological compound additive comprises Saccharomyces cerevisiae MST01 ( Saccharomyces cerevisiae MST01), the preservation number is CCTCC NO:M2020092, which can significantly improve the utilization rate of farmed animals to feed, promote the growth of farmed animals, improve meat quality, and can also improve the immunity of aquatic animals and the survival rate of the whole cycle, and can be widely used in aquatic feed field.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a strain of Saccharomyces cerevisiae and its application in aquaculture. Background technique [0002] With the increase in demand for aquatic products and the international development of aquatic product trade, the aquaculture industry has developed by leaps and bounds in recent years. However, the intensification and high-density scale of aquaculture is expanding day by day, the deterioration of the aquaculture environment and the harm of diseases have brought huge economic losses to the aquaculture industry. [0003] Litopenaeus vannamei occupies a large proportion in my country's aquaculture industry due to its adaptability to a wide range of salinity. With the rapid development of Litopenaeus vannamei aquaculture, the diseases of Litopenaeus vannamei are becoming more and more serious, and antibiotics have become the traditional preferred solution...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18A23K50/80A23K10/18A23K20/163A23K20/174C12R1/865
CPCA23K10/18A23K20/163A23K20/174A23K50/80C12N1/18
Inventor 王磊周怡刘广郭本月魏万权倪梦丽
Owner 青岛玛斯特生物技术有限公司
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