A combined use of HFDSPCS-CM and HA to promote diabetic wound healing

A technology for wound healing and diabetes, applied in biochemical equipment and methods, cell dissociation methods, metabolic diseases, etc., to achieve simple operation, good wound healing, and the effect of promoting wound healing

Active Publication Date: 2022-05-13
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Abstract
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  • Claims
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Problems solved by technology

[0007] So far, no research has reported the effect of human foreskin-derived dermal stem / progenitor cells conditioned medium (hFDSPCs-CM) combined with HA on diabetic wounds

Method used

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  • A combined use of HFDSPCS-CM and HA to promote diabetic wound healing
  • A combined use of HFDSPCS-CM and HA to promote diabetic wound healing
  • A combined use of HFDSPCS-CM and HA to promote diabetic wound healing

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Embodiment Construction

[0064] In order to enable those skilled in the art to better understand the technical solution of the present invention, its specific implementation will be described in detail below in conjunction with the accompanying drawings:

[0065] see figure 1 , the most preferred embodiment of the present invention, a method of jointly utilizing hFDSPCs-CM and HA to promote diabetic wound healing, comprising the following steps:

[0066] S1, cell acquisition and cultivation, specifically includes the following procedures:

[0067] (1) Extraction of hADSCs: Digest the discarded fat particles after liposuction with collagenase digestion method, extract adipose stem cells, and passage after confluence. The 0-3 passage (P1-P3) cells are used for experiments;

[0068] (2) Extraction of hFDSPCs: Digest children's foreskin tissue with neutral protease and collagenase, culture cells by cell cloning method, and the 0-3 generation (P1-P3) cells are used for experiments;

[0069] (3) Extractio...

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Abstract

The invention discloses a method for promoting the healing of diabetic wounds by using hFDSPCs-CM and HA, combining the conditioned medium of dermal stem cells derived from foreskin and hyaluronic acid to promote the healing of diabetic wounds, and for the first time the dermal stem cells / progenitor cells derived from The conditioned medium combined with hyaluronic acid is applied to the diabetic mouse model. Compared with the currently widely used conditioned medium of adipose-derived stem cells, it has a better effect of promoting wound healing, especially in promoting collagen synthesis. Diabetic refractory wounds are of great significance, and the simple operation is expected to achieve clinical transformation in the future. The present invention provides relevant data that the conditioned medium of the foreskin-derived dermal stem / progenitor cells promotes the healing of diabetic wounds, and explores the relevant mechanism in in vivo and in vitro experiments.

Description

technical field [0001] The invention relates to a method for promoting diabetic wound healing by combined use of hFDSPCs-CM and HA. Background technique [0002] Diabetic wounds are a common clinical problem characterized by insufficient chemokines and vascularization, decreased fibroblast migration and proliferation, and abnormal inflammatory responses. Current conventional treatments for this problem include customized excipients, surgical debridement, negative pressure therapy, antibiotics, and hyperbaric oxygen therapy. However, nearly 50% of patients still cannot be cured by traditional treatment methods. [0003] In recent years, a large number of studies have proved that mesenchymal stem cells from various tissue sources can promote diabetic wound healing, such as: bone marrow, fat, umbilical cord blood and skin tissue. Although mesenchymal stem cells from different tissue sources have certain similarities, they have different characteristics in different microenvir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077C12N5/0775C12Q1/02G01N33/68C12Q1/6888A61K35/28A61K31/728A61P17/02A61P3/10
CPCC12N5/0667C12N5/0668C12N5/0656G01N33/5008G01N33/5029G01N33/5044G01N33/6863C12Q1/6888A61K35/28A61K31/728A61P17/02A61P3/10C12N2509/00A61K2300/00
Inventor 章一新辛宇
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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