Method for degrading heavy metals in sludge by using extreme microorganisms, product and application
An extremophile and heavy metal technology, applied in the field of microbial engineering, can solve problems such as environmental secondary pollution and resource utilization problems, and achieve the effects of improving soil fertility, restoring and protecting soil ecology, and improving soil physical and chemical properties and biological activity
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Embodiment 1
[0049] A method for degrading heavy metals in sludge by extremophiles, comprising the following steps:
[0050] (1) Cultivation of extreme flora and fermentation flora: first screen and isolate the flora, and then use CMC to identify the culture medium and separate them from heavy metal industrial pollution areas. Cellulose can be produced at low temperature, room temperature, high temperature or extreme environments above 100°C Enzyme fungi and bacteria, the strains were inoculated in two kinds of straw disintegration media with different nitrogen sources, and cultured at 0-15°C, 15-40°C, 45-75°C and extreme environments for 58h, 65h, 80h, 90h, measure the size of the hydrolysis zone of the strain; select the strains with a hydrolysis zone larger than 1.5cm in each environmental range as low-temperature active bacteria, room temperature active bacteria, thermophilic strains and extreme bacteria; the four groups of bacteria were cultured separately in the culture medium , cult...
Embodiment 2
[0057] A method for degrading heavy metals in sludge by extremophiles, comprising the following steps:
[0058] (1) Cultivation of extreme flora and fermentation flora: first screen and isolate the flora, and use CMC to identify the culture medium and separate them from heavy metal industrial pollution areas. Fiber can be produced in extreme environments of low temperature, room temperature, high temperature or 20% salinity The fungus and bacteria of suzyme were inoculated in two kinds of straw disintegration media with different nitrogen sources, and cultured at 0-15°C, 15-40°C, 45-75°C and extreme environments for 48h, 96h, and 55h respectively , 90h, measure the size of the hydrolysis zone of the strain; select the strains with a hydrolysis zone greater than 1.5cm in each environmental range as low-temperature active bacteria, room temperature active bacteria, thermophilic strains and extreme bacterial groups; the four groups of bacteria were separately carried out in the cu...
Embodiment 3
[0065] A method for degrading heavy metals in sludge by extremophiles, comprising the following steps:
[0066] (1) Cultivation of extreme flora and fermentation flora: first screen and isolate the flora, and use CMC to identify the culture medium and separate them from heavy metal industrial pollution areas. Cellulose can be produced in extreme environments such as low temperature, room temperature, high temperature or pH<2 Enzyme fungi and bacteria, the strains were inoculated in two straw disintegration media with different nitrogen sources, and cultured at 0-15°C, 15-40°C, 45-75°C and extreme environments for 60h, 48h, 96h, 50h, measure the size of the strain's hydrolysis circle; select the strains with a hydrolysis circle greater than 1.5cm in each environmental range as low-temperature active bacteria, room temperature active bacteria, thermophilic strains and extreme bacteria; the four groups of bacteria were cultured separately in the culture medium , cultivated to the...
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