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A construction method and database of unknown plant species identification database based on matk gene

A construction method and database technology, which is applied in the field of plant species identification, can solve the problems of lack of accurate, efficient, and coverage databases, and achieve high sensitivity and selectivity, high data quality, and high detection throughput.

Active Publication Date: 2022-06-17
EZHOU INST OF IND TECH HUAZHONG UNIV OF SCI & TECH +1
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Problems solved by technology

[0007] The invention provides a construction method and database of an unknown plant species identification database based on the matK gene, which solves the problem in the prior art that there is still a lack of an accurate, efficient, and large-coverage database when identifying unknown plant species

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  • A construction method and database of unknown plant species identification database based on matk gene
  • A construction method and database of unknown plant species identification database based on matk gene
  • A construction method and database of unknown plant species identification database based on matk gene

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[0062] The present invention will be described in detail below with reference to specific embodiments and examples, and the advantages and various effects of the present invention will be more clearly presented therefrom. It should be understood by those skilled in the art that these specific embodiments and examples are used to illustrate the present invention, but not to limit the present invention.

[0063] Throughout the specification, unless specifically stated otherwise, terms used herein are to be understood as commonly used in the art. Therefore, unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present specification takes precedence.

[0064] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or can be prepared...

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Abstract

A construction method and database of an unknown plant species identification database based on the matk gene, the method comprising the steps of: obtaining an original sequence data file containing the matk gene; extracting species annotation information in the original sequence data file; The annotation information is integrated into the matK gene to obtain the matK sequence; quality control is performed on the matk sequence; clustering is performed according to the similarity between the matk sequences; and the database is constructed according to the clustering results. The construction method and database advantages of a kind of unknown plant species identification database based on matk gene provided by the application are: (1) high detection throughput; (2) high sensitivity and selectivity; (3) database coverage constructed by the method (4) Bioinformatics methods can be used to identify unknown species at the species level.

Description

technical field [0001] The invention belongs to the field of plant species identification, in particular to a construction method and a database of an unknown plant species identification database based on matk gene. Background technique [0002] The matK gene exists in the chloroplast lysine trnK gene and is located in an intron between two highly conserved exons. It is a single-copy coding gene and is involved in the cleavage of the coding transcript by the type II intron. Mature enzyme, the evolution rate is between rbcL and ITS, and has high species identification ability at the family and genus level. In 2009, the matK gene and the rbcL gene were recommended by the Consortium for Biological Barcoding (CBOL) as the core DNA barcodes for plant DNA barcode-related research based on the criteria of sequencing quality, species identification efficiency, and versatility. [0003] Although the matk gene has excellent species identification efficiency, versatility, and sequenc...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16B20/00G06K9/62G16B50/00
CPCG16B20/00G16B50/00G06F18/23
Inventor 宁康白虹杨朋硕卢璟详邹欣桐李洪军
Owner EZHOU INST OF IND TECH HUAZHONG UNIV OF SCI & TECH
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