Acaudina molpadioides and camel blood hypoglycemic peptide

A camel blood and hypoglycemic technology, which is applied in the field of Haitian melon and camel blood hypoglycemic peptides, can solve the problems of resource waste, poor taste, discarding, etc., and achieve good activity, excellent hypoglycemic effect, and good taste

Active Publication Date: 2020-09-01
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] As a substitute for sea cucumber, Haitian melon is cheap, rich in various amino acids and trace elements, and rich in nutrition, but it is not recognized by the public because of its poor taste
As a by-product of the camel industry chain, camel blood is often discarded, resulting in waste of resources and environmental pollution

Method used

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  • Acaudina molpadioides and camel blood hypoglycemic peptide
  • Acaudina molpadioides and camel blood hypoglycemic peptide
  • Acaudina molpadioides and camel blood hypoglycemic peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Thaw 1L of fresh camel blood or frozen camel blood, filter it with a filter screen of 4-400 meshes after homogenization to obtain pretreated camel blood, add 2% compound protease (Aspergillus niger protease: trypsin) to the pretreated camel blood =1:1), wherein, Aspergillus niger protease was purchased from Nanning Dongheng Huadao Biotechnology Co., Ltd. (production license number: SC10345010800150), the enzymolysis time was 4h, the temperature was 50°C, and the pH value was 7; used in the enzymolysis process Stir with a magnetic stirrer. After enzymolysis, the enzymolysis solution is filtered through 1000Da and 160Da membranes to collect a concentrated solution with a molecular weight of 160-1000Da. The concentrated solution is decolorized and deodorized by activated carbon, and spray-dried to obtain 9.25g camel blood polypeptide.

[0027] The half maximal inhibitory concentration (IC) of the camel blood polypeptide obtained above to α-amylase 50 ) is 0.5mg / mL, the IC ...

Embodiment 2

[0035] 100g dried Haitian melon is pulverized, and water is added to make suspension, wherein the mass ratio of Haitian melon and water is 1:6; Then add the compound enzyme of 2% trypsin and papain (the mass ratio of trypsin and papain is 3 :2) Enzymolysis, using a magnetic stirrer during the enzymolysis process, the enzymolysis time is 2 hours, the temperature is 50° C., and the pH value is 7. After the enzymatic hydrolysis, add ethanol to precipitate the polysaccharide, centrifuge at 8000rpm for 20min, take the supernatant, filter the supernatant through 1000Da and 360Da membranes, collect the concentrated solution with a molecular weight of 360-1000Da, decolorize the concentrated solution with activated carbon, remove fishy smell, After spray drying, 13.5 g of Haitian melon polypeptide was obtained.

[0036] After pretreatment of 1L camel blood, add 2% Aspergillus niger protease and trypsin (2:1) for enzymatic hydrolysis, use a magnetic stirrer during the enzymatic hydrolys...

Embodiment 3

[0041] Crush 100g of dried Haitian melon, add water to make a suspension, wherein the mass ratio of Haitian melon to water is 1:6; then add 4% trypsin and papain compound enzyme (3:1) for enzymatic hydrolysis, the enzymatic hydrolysis process Use a magnetic stirrer in the medium, enzymolysis time 2h, temperature 50°C, pH value 7; after enzymolysis, add ethanol to precipitate polysaccharides, centrifuge at 8000rpm for 20min, take the supernatant, and filter the supernatant through 1000Da and 360Da membranes , collect the concentrated solution with a molecular weight of 360-1000, decolorize the concentrated solution with activated carbon, remove the smell, and spray dry to obtain 17.8g of Haitian melon polypeptide.

[0042] After pretreatment of 1L camel blood, add 2% Aspergillus niger protease and trypsin (1:2) for enzymatic hydrolysis, use a magnetic stirrer during the enzymatic hydrolysis, enzymatic hydrolysis time 4h, temperature 50°C, pH value 7; enzymatic hydrolysis After ...

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Abstract

The invention provides a preparation method of acaudina molpadioides and camel blood hypoglycemic peptide. The acaudina molpadioides and camel blood hypoglycemic peptide comprises the following components in parts by weight: 1-9 parts of acaudina molpadioides polypeptide and 1-9 parts of camel blood polypeptide. The acaudina molpadioides and the camel blood are used as raw materials; the acaudinamolpadioides hypoglycemic peptide and the camel blood hypoglycemic peptide are prepared through protease enzymatic hydrolysis, membrane separation, and other technologies, the acaudina molpadioides hypoglycemic peptide and the camel blood hypoglycemic peptide are mixed in a proper proportion, and the acaudina molpadioides and camel blood hypoglycemic peptide is prepared and can reduce blood sugar.The preparation method is green and environment-friendly, realizes high-valued utilization of camel blood and acaudina molpadioides, has a wide market prospect, and can generate good economic and social benefits.

Description

technical field [0001] The invention belongs to the technical field of active substance development, and in particular relates to a hypoglycemic peptide of Haitian melon and camel blood. Background technique [0002] Diabetes is a group of metabolic diseases characterized by hyperglycemia. The prevalence of diabetes in my country ranks first in the world. The incidence of type 2 diabetes accounts for 10.4% of the adult population over 18 years old, and the prevalence rate of the elderly over 60 years old is as high as 20%. Diabetes can lead to chronic damage and dysfunction of various tissues, especially eyes, kidneys, heart, blood vessels, and nerves. If diabetes cannot be effectively controlled for a long time, it can lead to serious consequences such as stroke, coronary heart disease, blindness, renal failure, uremia, and necrosis of lower limbs. The cause of diabetes has a certain relationship with lifestyle and bad living habits. Patients with diabetes should be treat...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 邸多隆王宁丽裴栋魏鉴腾
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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