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Method for eucalyptus tissue culture explant preparation and sterile bud induction

A bud induction and explant technology, applied in the field of plant reproduction, can solve the problems of large economic loss, high pollution rate in the explant induction stage, low efficiency of obtaining sterile buds, etc., so as to prevent the browning of new shoots and improve the utilization rate. , The effect of preventing shoots from growing bacteria

Active Publication Date: 2020-07-14
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The industrial production of eucalyptus tissue culture seedlings is developing rapidly in the south of my country, and the rapid propagation technology is becoming more and more mature. However, the pollution rate in the explant induction stage is high, and the acquisition efficiency of sterile buds is low, resulting in large economic losses. Therefore, how to overcome Pollution is a technical link that cannot be ignored

Method used

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  • Method for eucalyptus tissue culture explant preparation and sterile bud induction
  • Method for eucalyptus tissue culture explant preparation and sterile bud induction
  • Method for eucalyptus tissue culture explant preparation and sterile bud induction

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Effect test

Embodiment 1

[0036] A method for preparing eucalyptus tissue culture explants and inducing sterile buds, the main steps of which are as follows:

[0037] (1) Excellent tree selection

[0038] Select the top 5% of the growth indicators such as tree height, diameter at breast height, and single plant volume in the sample plot, and the eucalyptus individuals with no pests and diseases, straight trunk shape, and small branches are selected as excellent trees;

[0039] (2) Circumcision to promote germination

[0040] Eradicate miscellaneous shrubs around the excellent tree, spray 75% alcohol around the excellent tree one day before the circumcision for disinfection, and perform circumcision at 3 / 4 of the circumference of the excellent tree at a distance of 20-25cm from the ground, with a width of 2 cm and peel the bark And scrape out the cambium to the phloem;

[0041] (3) Hydroponics to promote germination

[0042] When the sprouts grow to a diameter of 2-3cm, cut off 60cm-long, completely ...

Embodiment 2

[0055] A method for preparing eucalyptus tissue culture explants and inducing sterile buds, the main steps of which are as follows:

[0056] (1) Excellent tree selection

[0057] Select the top 5% of the growth indicators such as tree height, diameter at breast height, and single plant volume in the sample plot, and the eucalyptus individuals with no pests and diseases, straight trunk shape, and small branches are selected as excellent trees;

[0058] (2) Circumcision to promote germination

[0059] Eradicate miscellaneous shrubs around the excellent tree, spray 75% alcohol around the excellent tree one day before the circumcision for disinfection, and perform circumcision at 3 / 4 of the circumference of the excellent tree at a distance of 20-25cm from the ground, with a width of 2 cm and peel the bark And scrape out the cambium to the phloem;

[0060] (3) Hydroponics to promote germination

[0061] When the sprouts grow to a diameter of 2-3cm, cut off 60cm-long, completely ...

Embodiment 3

[0074] A method for preparing eucalyptus tissue culture explants and inducing sterile buds, the main steps of which are as follows:

[0075] (1) Excellent tree selection

[0076] Select the top 5% of the growth indicators such as tree height, diameter at breast height, and single plant volume in the sample plot, and the eucalyptus individuals with no pests and diseases, straight trunk shape, and small branches are selected as excellent trees;

[0077] (2) Circumcision to promote germination

[0078] Eradicate miscellaneous shrubs around the excellent tree, spray 75% alcohol around the excellent tree one day before the circumcision for disinfection, and perform circumcision at 3 / 4 of the circumference of the excellent tree at a distance of 20-25cm from the ground, with a width of 2 cm and peel the bark And scrape out the cambium to the phloem;

[0079] (3) Hydroponics to promote germination

[0080] When the sprouts grow to a diameter of 2-3cm, cut off 60cm-long, completely ...

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Abstract

The invention discloses a method for eucalyptus tissue culture explant preparation and sterile bud induction. The method comprises procedures of superior tree selection, ringing germination promotion,water culture germination promotion, explant treatment and induction culture, namely selecting a superior tree of eucalyptus; performing ringing germination promotion at 20-25cm from the ground; whengerminated twigs grow to 2-3cm in diameter, cutting twigs which are 60cm in length and are completely lignified with latent buds, and performing water culture in a culture chamber; when the latent buds germinate and grow to 3-4cm, cutting stems as explants, performing sterilization, inoculating the explants in an induction culture medium, and performing induction culture in an appropriate environment, so as to obtain eucalyptus sterile buds which grow strongly and healthily with activity and vigorousness. By adopting the method, the eucalyptus tissue culture explant induction contamination rate can be reduced, and the sterile bud yield is high.

Description

technical field [0001] The invention belongs to the technical field of plant propagation and relates to tissue culture seedling raising technology, in particular to a method for preparing tree tissue culture explants and inducing sterile buds. Background technique [0002] Eucalyptus is a general term for three genera of trees belonging to the family Myrtaceae (Angophora), Corymbia (Corymbia) and Eucalyptus (Eucalyptus). There are 808 species and 137 subspecies or varieties, a total of 945 taxa. The natural distribution is east of the Wallace line, from 7° north latitude to 43° 39′ south latitude. Except for Eucalyptus stripped in Papua New Guinea, Indonesia and the Philippines, and Eucalyptus urophylla, Eucalyptus urophylla and Eucalyptus urophylla distributed in Timor Island and the eastern islands of Indonesia, the rest are naturally distributed in Australia. [0003] Most eucalyptus are easy to reproduce asexually. Since 1980, my country has carried out cutting and tis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C05G3/60C05G5/20
CPCA01H4/008A01H4/001A01G31/00C05F11/10C05G3/00Y02P60/21
Inventor 邓紫宇刘媛卢翠香任世奇郭东强唐庆兰陈升侃伍琪李昌荣周维陈健波甘春雁
Owner GUANGXI FORESTRY RES INST
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