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A kind of method for establishing the tissue culture system of body odor Chai

A technique of tissue culture and Chai skunk, which is applied in the field of establishment of a tissue culture system of Chai skunk, which can solve the problems of poor resistance to cutting propagation and easy degradation, and meet the needs of Chai slug seedlings, improve the quality of the seedlings, and solve the seasonal limitations Effect

Active Publication Date: 2021-10-22
GUIZHOU UNIV
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Problems solved by technology

[0003] At present, Chai bodhisattva is mainly propagated by cuttings, but the cuttings have poor resistance and are prone to degeneration after multi-generation propagation, and there are also requirements for cutting time, which has certain limitations, so different propagation methods are sought to make up for this shortcoming

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  • A kind of method for establishing the tissue culture system of body odor Chai
  • A kind of method for establishing the tissue culture system of body odor Chai
  • A kind of method for establishing the tissue culture system of body odor Chai

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Embodiment Construction

[0022] The present invention will be further described below in conjunction with the accompanying drawings and embodiments, but not as a basis for limiting the present invention.

[0023] Example. A kind of method that establishs body odor Chai tissue culture system, constitutes such as Figure 1-4 As shown, the plant material Premnapuberula Pamp. in this example was collected from Wuchuan Gelao and Miao Autonomous County, Guizhou Province, and was identified by Professor Xiong Yuanxin of Guizhou University.

[0024] Step 1: Disinfection of explants: Select the stem section from the upper end of the young shoots of Chai bodhisattva with excellent agronomic characteristics in the field, trim the stem section to a length of about 1 cm after removing the leaves, put it in a beaker and rinse it under running water for 1-2 hours, drain After the water is dried, the surface is disinfected with alcohol, placed in a sterile operating table, treated with 75% alcohol for 45 seconds, rin...

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Abstract

The invention discloses a method for establishing a tissue culture system of the body odor. The method includes the following steps: taking the stem section of Chai bodhisattva as an explant, and disinfecting the surface; cutting the sterilized explant into sections, inoculating it in a callus induction medium and culturing it to obtain a callus; The callus is transferred to the clustering bud induction medium and cultivated to grow clustered buds; the clustering buds are connected in the strong seedling medium to carry out the elongation and strong seedling cultivation of the clustering buds, until the clustering buds grow to 2-3cm, and the clustering buds are obtained seedlings; the clustered shoots were transferred to the rooting medium for cultivation to obtain the rooted shoots of Chai chinensis. In the present invention, through callus induction on the axillary bud stems of the body odor, a large number of body odor seedlings suitable for transplanting can be cultivated in a short period of time, which can significantly improve the seedling quality of the body odor, and effectively solve the seasonal limitation of the body odor cutting propagation It can achieve large-scale production and meet the beneficial effects of the market demand for the body odor Chai seedlings.

Description

technical field [0001] The invention relates to a method for tissue culture system, in particular to a method for establishing the tissue culture system of Chai odorifera. Background technique [0002] Premnapuberula (Premnapuberula Pamp.), also known as Immortal Tofuchai, is an upright or climbing shrub to small tree belonging to the genus Tofuchai in the Verbenaceae family. Under natural conditions, it is difficult to germinate the seeds of P. chinensis, and its wild resources are scattered. Therefore, artificial planting is very necessary to realize the industrial development of P. chinensis resources. [0003] At present, Chai bodhisattva is mainly propagated by cuttings, but the cuttings have poor resistance and are prone to degeneration after multi-generation propagation, and there are also requirements for cutting time, which has certain limitations, so different propagation methods are sought to make up for this shortcoming . Therefore, through the establishment of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张明生幸菲菲刘剑东李扬阳娇王艳秋
Owner GUIZHOU UNIV
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