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Composition for ameliorating human skin cell damage caused by ultraviolet rays containing hydrangea chinensis extract

A technology of skin cells and extracts, applied in the direction of medical preparations containing active ingredients, skin care preparations, applications, etc., can solve problems such as the mechanism research of human epidermal cells and dermal cells, to prevent skin aging and inhibit secretion , increase the effect of secretion

Active Publication Date: 2021-08-13
COSMAX BIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there is no direct research on the mechanism by which hydrangea rough tooth inhibits human epidermal cells and dermal cells caused by ultraviolet rays.

Method used

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  • Composition for ameliorating human skin cell damage caused by ultraviolet rays containing hydrangea chinensis extract
  • Composition for ameliorating human skin cell damage caused by ultraviolet rays containing hydrangea chinensis extract
  • Composition for ameliorating human skin cell damage caused by ultraviolet rays containing hydrangea chinensis extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Preparation of extracts utilizing ethanol

[0036]The hydrangea crassa extract contained in the composition of the present invention is prepared by the following procedure. First, buy a one-year-old hydrangea that grows on Jeju Island, wash it with distilled water, and dry it completely in a cool place without sunlight until there is no change in weight. After that, the completely dried dry product was pulverized with a homogenizer to obtain the pulverized product, and then 50 g of the pulverized product was added after mixing ethanol:distilled water at a weight ratio of 7:3 to prepare 400 g of each extraction solvent. At this time, the mixing ratio of the pulverized product and the extraction solvent was 1:8 by weight. Next, the extraction solvent mixed with the pulverized product was put into a shaking constant temperature water tank, and stirred and extracted at 50° C. for 2 hours. The substances obtained by extraction were extracted with Whatman (No 2....

experiment example 1

[0037] Experimental example 1: Cytotoxicity evaluation of the ethanol extract of Hydrangea coarse tooth

[0038] It was determined whether the extract obtained in Example 1 above had cytotoxicity to skin cells. In this experiment, HaCaT Keratinocytes were used as keratinocytes, and HS68 fibroblasts were used as dermal cells. In order to confirm whether there is cytotoxicity, 1.0X10 4 The individual cells of the number of cells were dispensed on a 96-well plate, adhered for 24 hours, and stabilized, and then treated with ethanol extracts of Hydrangea dentata at concentrations of 0, 5, 25, and 125 ppm (μg / ml). After 24 hours, the degree of cell viability was determined by MTT assay. The MTT assay is a method for measuring the amount of formazan formed from MTT reduced by the mitochondria of surviving cells. Each cell was treated with 50 µl of a 5 mg / ml MTT solution, and after culturing for 4 hours, the solution was completely removed, and the plate dissolved in dimethyl sul...

experiment example 2

[0042] Experimental example 2: Evaluation of recovery from cell damage caused by UV-B of the ethanol extract of Hydrangea japonica

[0043] With respect to the extract obtained in Example 1 above, it was determined whether the damage of skin cells exposed to UV-B could be restored. In this experiment, HaCaT Keratinocyte was used as keratinocytes, and HS68fibroblast was used as dermal cells. In order to confirm whether there is a cell recovery effect, 1.0X10 4 Each cell of the cell number was dispensed on a 96-well plate, adhered for 24 hours, and after stabilization, 15mJ / cm 2 UV-B irradiation. Afterwards, the medium was replaced with the medium treated with the ethanol extract of Hydrangea crachydenta at concentrations of 0, 5, 25, and 125 ppm (μg / ml), and the degree of cell survival was determined by MTT assay. The MTT assay is a method for measuring the amount of formazan formed from MTT reduced by the mitochondria of surviving cells. More specifically, each cell was ...

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Abstract

The present invention relates to a composition for improving damage of human epidermal cells and dermal cells caused by ultraviolet rays, which contains an extract of Hydrangea crachydenta as an active ingredient. According to the present invention, the composition of the present invention can not only restore the cell damage caused by UV-B in human epidermal cells and dermal cells, but also increase the secretion of hyaluronic acid and inhibit the secretion of MMP-1, thereby improving Cellular damage caused by UV‑B. Therefore, the hydrangea crassa extract, which is a safe raw material, can be usefully used in the field of food compositions or in the field of cosmetics.

Description

technical field [0001] The present invention relates to a composition for improving human skin cell damage caused by ultraviolet rays, and more particularly to a composition for improving human epidermal cell and dermal cell damage caused by UV-B exposure, comprising Hydrangea crachydera extract as an active ingredient. Background technique [0002] Skin aging can be divided into intrinsic aging and extrinsic aging according to factors. It is reported that the cause of endogenous aging is the physiological function changes of the skin epidermis and dermis that appear with age, and the cause of exogenous aging is the physiological function of the skin that occurs in the environment such as air pollution, ultraviolet exposure, and stress. Variety. In this aging mechanism, the oxidative stress (Oxidative Stress) induced by UV increases the free radicals (Free Radical) in the body, and the matrix metalloproteinase-1 (MMP-1, matrixmetalloproteinase-1) that breaks down collagen (...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9789A61K36/185A23L33/105A61Q17/04A61Q19/00
CPCA23V2002/00A23V2200/318A61K36/185A61Q17/04A61Q19/00A23L33/105A61K8/9789
Inventor 李槿硕金贤宰李仙姬金知亨
Owner COSMAX BIO
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