Cells for targeted treatment of habitual abortion and immune infertility and preparation method of cells

Abstract

The invention belongs to the technical field of biomedicine, and discloses cells for targeted treatment of habitual abortion and immune infertility and a preparation method of the cells. The preparation method of the cells for the targeted treatment of the habitual abortion and the immune infertility includes the following steps: aseptically drawing about 20 ml of venous blood of a patient husbandor blood relative or unrelated voluntary healthy individual, and performing heparin anticoagulation; under sterile conditions, adding an equal volume of human lymphocyte separation solution to a centrifuge tube, slowly adding the anticoagulant whole blood to the upper layer of the human lymphocyte separation solution, and performing centrifugation at 4 DEG C and 800-1200 rpm in a slow rise and fall manner; and drawing a separated buffy coat by using a pipette, performing washing by using normal saline, performing resuspension, performing centrifugation at 800-1200 rpm, repeating the operationfor 3 times, removing residual serum, red blood cells and the like, preparing a cell suspension with a density of (2-4)x10<7> / ml, and performing temporary storage at 4 DEG C. The cells improve the conception rate, reduce the miscarriage rate, and make the pregnancy success rate reach 70-80% or more.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a cell for targeted treatment of habitual abortion and immune infertility and a preparation method thereof. Background technique [0002] Currently, the closest prior art: Infertility is divided into infertility and infertility. Couples of childbearing age who live together for more than one year, have normal sex life, and fail to conceive without any contraceptive measures are called infertility. Although it can conceive, it is called infertility that can not obtain a surviving baby due to miscarriage or stillbirth due to various reasons. The infertility of the spouse due to male reasons is called male infertility or male infertility, and it is customary to call it male infertility. Habitual miscarriage, also known as recurrent miscarriage, refers to those who have had two or more spontaneous miscarriages, and each miscarriage often occurs in the same month of ...

AI Technical Summary

Problems solved by technology

[0009] Difficulty in solving the above-mentioned technical problems: For habitual abortion, the existing technology mainly adopts resting in bed, prohibiting sexual life, repairing internal os of the cervix, cervical cerclage, supplementing hormones, and vitamin drugs to prevent habitual abortion. There is no better way than proper use of traditional Chinese medicine

It cannot fundamentally solve the occurrence of miscarriage or infertility caused by immune factors such as the lack of blocking antibodies

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