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Detection reagent for detecting adenosine triphosphate, preparation method of detection reagent, and method and device for detecting adenosine triphosphate by using detection reagent

A technology of adenosine triphosphate and detection reagents, applied in the field of analytical chemistry, can solve problems such as poor anti-interference ability, detection of adenosine triphosphate, and low sensitivity

Active Publication Date: 2020-06-09
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in related technologies, the method for detecting the content of ATP has poor anti-interference ability and low sensitivity; at the same time, it is impossible to achieve rapid, real-time and continuous detection of ATP
[0004] Therefore, the existing methods for detecting ATP content still need to be improved

Method used

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  • Detection reagent for detecting adenosine triphosphate, preparation method of detection reagent, and method and device for detecting adenosine triphosphate by using detection reagent
  • Detection reagent for detecting adenosine triphosphate, preparation method of detection reagent, and method and device for detecting adenosine triphosphate by using detection reagent
  • Detection reagent for detecting adenosine triphosphate, preparation method of detection reagent, and method and device for detecting adenosine triphosphate by using detection reagent

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Experimental program
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Effect test

Embodiment 1

[0083] Method for preparing detection reagents:

[0084] Add 9 mg of laccase to 3 mL of an aqueous solution of 0.4 mM imidazole-2-carbaldehyde under stirring conditions to obtain a first mixture; then add an aqueous solution of zinc acetate dihydrate to the first mixture to obtain a second mixture, and make The concentration of zinc acetate dihydrate in the second mixture is 0.01 mM; the second mixture is stirred at a speed of 600 r / min for 10 minutes, and then centrifuged, washed and dried to obtain the first reagent, and collected The supernatant obtained after centrifugation as described above. The first reagent was redispersed into the artificial cerebrospinal fluid with a pH value of 7.4, and the obtained solution was a solution of the first reagent at a concentration of 1 mg / mL.

[0085] 0.95 mg of the second reagent, ie, dopamine, was dissolved in 10 mL of deionized water to obtain a solution with a concentration of 500 μM of the second reagent.

[0086] For the trans...

Embodiment 2

[0096] The method of using the detection reagent to detect the content of adenosine triphosphate in the sample to be tested:

[0097] The solution of the first reagent provided in Example 1, the solution of the second reagent and the artificial cerebrospinal fluid are fed continuously and synchronously in the manner described above, such as Figure 4 In the device shown in Figure B, the background current value I 0 , in this embodiment, both the first reactor and the second reactor are capillary tubes.

[0098] To be the background current value I 0 After stabilization, replace the artificial cerebrospinal fluid with 6 parts of standard solutions of adenosine triphosphate of different concentrations, and mix with the solution of the first reagent and the solution of the second reagent in turn to pass into the thin-layer flow electrolytic cell, wherein the concentrations of the standard solutions of adenosine triphosphate are respectively 2nM , 10nM, 50nM, 100nM, 500nM, 1000n...

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Abstract

The invention provides a detection reagent for detecting the content of adenosine triphosphate, a preparation method of the detection reagent, and a method and a device for detecting the content of adenosine triphosphate by using the detection reagent. The detection reagent comprises a first reagent and a second reagent, wherein the first reagent comprises ZIF-90 crystal grains and laccase, the laccase is embedded in the crystal structure of the ZIF-90 crystal grain, and the second reagent comprises dopamine. The detection reagent is simple in preparation process, readily available in raw materials, relatively low in cost, and easy in industrial production; when the detection reagent is used for detecting the content of adenosine triphosphate, the detection reagent has particularly outstanding anti-interference capability, has the advantages of wide linear range, complete coverage of the physiological concentration range of adenosine triphosphate, low detection limit, high sensitivity,strong stability and good reproducibility, can realize rapid, real-time and continuous detection of adenosine triphosphate in a to-be-detected sample, and is especially suitable for detection of adenosine triphosphate in the physiological process of living brain nerves.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, in particular to a detection reagent for detecting adenosine triphosphate, a preparation method thereof, a method and a device for detecting adenosine triphosphate using the same. Background technique [0002] At present, adenosine triphosphate is one of the indispensable biomolecules in living organisms. It has been widely recognized as the main source of chemical energy for biological substances and its role in cellular processes. It plays a vital role in regulating many cellular functions and processes, including acting as an energy provider, catalyzing various enzymes, maintaining Extracellular signal transduction mediator of cellular respiration, etc. Furthermore, in the context of the brain, the release of ATP is a key step in central chemosensory transmission. [0003] However, in related technologies, the method for detecting the content of ATP has poor anti-interference ab...

Claims

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Application Information

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IPC IPC(8): C08L87/00C08L89/00C08K5/18C08G83/00G01N27/416
CPCC08G83/008C08L87/00C08L89/00G01N27/416C08K5/18
Inventor 林雨青李凯张望王超彭美红任国圆
Owner CAPITAL NORMAL UNIVERSITY
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