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A method for detecting fat traits of cattle by using sdc3 promoter genetic marker

A technology of molecular markers and gene promoter regions, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of few reports, improve population quality, improve accuracy and precision, and improve cattle succulent effect

Active Publication Date: 2021-03-23
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the SDC3 gene is focused on the gene function research in mice and humans, and there are few reports on the promoter research of the SDC3 gene and its SNPs in the study of beef quality traits.

Method used

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  • A method for detecting fat traits of cattle by using sdc3 promoter genetic marker
  • A method for detecting fat traits of cattle by using sdc3 promoter genetic marker
  • A method for detecting fat traits of cattle by using sdc3 promoter genetic marker

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Experimental program
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Effect test

Embodiment 1

[0039] 1. Experimental materials and equipment

[0040] 2×Taq PCR Green Mix (Novizyme, Nanjing), ethidium bromide substitute (Dingguochangsheng, Beijing), DL2000 DNA Marker (TaKaRa, Dalian), agarose (Biowest Agarose), 50×TAE (Sole precious).

[0041]PCR instrument (BIO-RAD Company); electrophoresis instrument (Shanghai Junyi Co., Ltd.); high-speed centrifuge (Shanghai Chemical Machinery Factory Co., Ltd.); -20 ℃ refrigerator (Haier); PCR tube (Axygen Company); gel imaging system (UVC);

[0042] 2. The main software used in the experiment

[0043] Sequencing results data analysis software SeqMan and statistical analysis software SPSS 23.0, etc. The linkage relationship and haplotype of 10 single nucleotide polymorphisms were analyzed using HaploView software;

[0044] 3. Experimental method

[0045] The blood of Chinese Simmental bulls was provided by Baolong Shanniu Farm in Inner Mongolia. Genomic DNA was extracted using a whole blood DNA extraction kit, and the DNA of ea...

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Abstract

The invention discloses a method for detecting bovine fat traits by utilizing an SDC3 promoter genetic marker. By carrying out PCR amplification on whole genome DNA of cattle and by detecting SNPs existing in a PCR product sequence by DNA sequencing, the invention discloses the correlation between seven SNPs sites on an SDC3 gene promoter region and bovine fat traits; and the method for predictingand breeding marbling grade, fat coverage rate and fat color character of beef cattle by utilizing the SDC3 gene promoter region marker lays a foundation for early breeding of genetic workers, improvement of meat quality and further improvement of the economic benefit of beef cattle breeding.

Description

Technical field: [0001] The invention discloses a method for detecting bovine fat traits by using SDC3 promoter genetic markers, in particular to a method for predicting bovine fat coverage, marbling, and fat color traits by detecting SNPs sites and haplotypes in the promoter region of SDC3 gene The molecular marker detection method and its application for evaluation and evaluation belong to the technical field of breeding prediction and breeding beef quality traits. Background technique: [0002] In the field of beef cattle feeding technology, the functional genes and molecular markers related to cattle fat traits are of great significance and application value especially for the early screening and breed improvement of beef cattle. [0003] Single nucleotide polymorphisms (SNPs) mainly refer to DNA sequence polymorphisms caused by single nucleotide variation at the genome level. The polymorphism represented by SNP only involves the variation of a single base, which can be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 白紫彤房希碧赵志辉杨润军于海滨夏立新余湘米家其孙豪
Owner JILIN UNIV
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