A fluorescent probe for detecting acetylcholinesterase activity and its synthesis method and application

Abstract

The invention discloses a fluorescent probe for detecting acetylcholinesterase activity and its synthesis method and application. The invention is based on MnO 2 The electrostatic interaction between the nanosheets and the cationic derivative of perylenetetracarboximide (PBI‑DM), as well as the fluorescence spectral characteristics of PBI‑DM during the aggregation and dispersion process, enable the detection of AChE activity. The invention discloses a synthetic fluorescent probe PBI-DM@MnO 2 The detection of AChE by NS has a low detection limit of 0.24mU / mL, and can avoid the interference of other sulfhydryl compounds at low concentrations, and has high selectivity. At the same time, PBI‑DM@MnO 2 NS can be used for AChE inhibitor screening and has great potential in the field of clinical diagnosis.

Description

technical field [0001] The invention belongs to the technical field of acetylcholinesterase activity detection, and relates to a method strategy for detecting acetylcholinesterase (AChE) activity. More specifically, the present invention not only relates to a MnO-based 2 Electrostatic interactions between nanosheets and cationic derivatives of perylenetetracarboximide (PBI-DM), and a new method for detecting AChE activity of PBI-DM's fluorescence spectral features during aggregation and dispersion, also related to the above Synthesis of fluorescent probes and their application in screening AChE inhibitors. Background technique [0002] Alzheimer's disease, one of the most common forms of dementia, is a chronic neurodegenerative disorder with symptoms including short-term memory loss, primary progressive aphasia, and confusion. Studies have shown that AChE can accelerate the abnormal aggregation of amyloid beta peptides to form Alzheimer's amyloid fibers in the central nerv...

AI Technical Summary

Problems solved by technology

Although the Ellman test is widely used, a large number of analytical studies have shown that it has certain shortcomings and limitations in the detection of AChE activity due to its false positive effect and poor precision.

In addition, conventional assays are less sensitive compared to luminescence assays, therefore, luminescence-sensing-based fluorescence assays have become the most attractive strategy for measuring AChE activity due to their ease of development, high sensitivity, good selectivity, and ease of operation. one

Images