Method for obtaining crassostrea hongkongensis monomers through medicine induction
A Hong Kong oyster and drug technology, which is applied in the field of drug-induced acquisition of Hong Kong oyster monomer, can solve the problems of low yield per unit water body, easy damage to oyster seedlings, and low survival rate, so as to improve the rate of non-attached metamorphosis of larvae and increase the rate of non-attached metamorphosis rate effect
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Embodiment 1
[0022] A method for drug-induced acquisition of Hong Kong oyster monomers, comprising the following steps:
[0023] (1) Larvae cultivation: Artificial insemination, hatching and larvae cultivation are carried out in accordance with the Hong Kong Oyster Seed Breeding Technical Regulations;
[0024] (2) Screening of foot disc larvae: when 52% of the larvae had eyespots and 34.5% of the larvae stretched their feet, collect the larvae and screen the larvae with an 80-mesh silk screen to obtain a foot disc larvae with a size of 320 μm;
[0025] (3) Drug induction: Induce drug induction on the disc larvae screened in step (2), add the disc larvae to the induction solution according to the ratio of adding 20 g of disc larvae to the induction solution of 500 mL, and then place Treat in the dark for 4 hours, during which air stones are put into the induction solution, and a small amount of air is continuously filled to keep the dissolved oxygen at 6 mg / L; the induction solution is an a...
Embodiment 2
[0030] A method for drug-induced acquisition of Hong Kong oyster monomers, comprising the following steps:
[0031] (1) Larvae cultivation: Artificial insemination, hatching and larvae cultivation are carried out in accordance with the Hong Kong Oyster Seed Breeding Technical Regulations;
[0032] (2) Screening of foot disc larvae: when 48.5% of the larvae had eyespots and 38.7% of the larvae stretched their feet, collect the larvae and screen the larvae with an 80-mesh silk screen to obtain a foot disc larvae with a size of 320 μm;
[0033] (3) Drug induction: Induce drug induction on the disc larvae screened in step (2), add the disc larvae to the induction solution according to the ratio of adding 20 g of disc larvae to the induction solution of 500 mL, and then place Treat in the dark for 4 hours, during which air stones are put into the induction solution, and a small amount of air is continuously filled to keep the dissolved oxygen above 5mg / L; the induction solution is ...
Embodiment 3
[0038] A method for drug-induced acquisition of Hong Kong oyster monomers, comprising the following steps:
[0039] (1) Larvae cultivation: Artificial insemination, hatching and larvae cultivation are carried out in accordance with the Hong Kong Oyster Seed Breeding Technical Regulations;
[0040] (2) Screening of foot disc larvae: when 50% of the larvae have eyespots and 40% of the larvae have stretched their feet, collect the larvae and screen the larvae with an 80-mesh silk screen to obtain a foot disc larvae with a size of 300 μm;
[0041] (3) Drug induction: Induce drug induction on the disc larvae screened in step (2), add the disc larvae to the induction solution according to the ratio of adding 20 g of disc larvae to the induction solution of 500 mL, and then place Treat in the dark for 4 hours, during which air stones are put into the induction solution, and a small amount of air is continuously filled to keep the dissolved oxygen above 5mg / L; the induction solution i...
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