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Molecular markers closely linked to wheat grain length major QTL and their application

A technology of molecular markers and wheat grains, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problem that molecular markers of grain length cannot be effectively used in molecular assisted breeding, and reduce the cost of phenotypic identification work, save breeding cost and improve breeding efficiency

Active Publication Date: 2020-06-30
LUDONG UNIVERSITY
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Problems solved by technology

Due to the differences in genetic populations and molecular markers used in research, some QTL intervals are relatively large, and their linkage markers are far away from the target gene, so molecular markers for grain length cannot be effectively used in molecular assisted breeding

Method used

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  • Molecular markers closely linked to wheat grain length major QTL and their application
  • Molecular markers closely linked to wheat grain length major QTL and their application
  • Molecular markers closely linked to wheat grain length major QTL and their application

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Embodiment Construction

[0035] The present invention will be specifically introduced below in conjunction with the accompanying drawings and specific embodiments.

[0036] 1. Molecular markers closely linked to major QTL for grain length in wheat

[0037] The wheat variety Kenong 9204 is a nationally approved variety in 2003, and the variety approval number is the nationally approved wheat 2003037.

[0038] Wheat variety Jing 411 is also an approved variety, which can be obtained from the National Crop Germplasm Resource Bank, and the national uniform number is ZM020984.

[0039] Using QTL mapping analysis, the grain length main effect QTL- qKl-1BL Located in the interval of AX-109370525~AX-110579468 on the long arm of chromosome 1B, using the resequencing data of Kenong 9204 and Jing 411, in qKl-1BL A polymorphic InDel molecular marker 1BL-16 was designed and developed near the segmental LOD prediction peak. The sequence of the upstream primer of the molecular marker 1BL-16 is: GGCCGCACAAAGGACA...

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Abstract

The present invention discloses a molecular marker closely linked with wheat grain length main effect QTL and its application. The molecular marker is based on wheat Jing 411 genomic DNA as a template, using the upstream primer shown in SEQ ID NO: 1 and SEQ ID The downstream primer shown in NO:2 carries out the DNA fragment obtained by PCR amplification, and its nucleotide sequence is shown in SEQ ID NO:3. Molecular markers provided by the present invention and qKl‑1BL Tight linkage can promote the transfer and application of this locus in popularized varieties, and is also conducive to the aggregation breeding of this locus and other yield-related loci; the molecular marker provided by the present invention is an Indel marker, which is co-dominant, and its primer pair has With the advantages of stable amplification, fast and efficient detection, etc., it can quickly determine whether there is qKl‑ 1BL and existing forms, and predict the grain length characteristics of wheat.

Description

technical field [0001] The invention relates to a molecular marker and an application thereof, in particular to a molecular marker closely linked with wheat grain length main effect QTL and an application thereof, and belongs to the technical field of crop seed selection and cultivation. Background technique [0002] wheat( Triticum aestivum L.) is one of the main food crops in the world and plays an important role in my country's food security. my country's available arable land area is small, which creates a huge contradiction with the grain demand. Therefore, it is necessary to increase the total grain output by increasing the unit yield on the limited land area. The number of effective ears, the number of grains per ear and the weight of grains are the three elements of wheat yield. Wheat grain type includes traits such as grain length, grain width and grain thickness, which are significantly positively correlated with wheat grain weight. In addition, wheat grain typ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 秦冉崔法荀海兰吴永振赵春华孙晗马天航周晓涵周芳婷
Owner LUDONG UNIVERSITY
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