Stem cell liquid preparation bin control system based on programmable controller and control method thereof

Abstract

The invention relates to a stem cell liquid preparation bin control system based on a programmable controller and a control method thereof. The system comprises an industrial computer, a bin body controller, a servo amplifier, an execution device, a peristaltic pump, a dust particle sensor and a planktonic bacteria sensor, and the industrial computer and the peristaltic pump are connected with thebin body controller; the bin body controller is connected with the servo amplifier through an SSCNET III network, the servo amplifier is connected with the execution device, a touch screen is connected with the bin body controller, and the bin body controller is connected with the dust particle sensor and the planktonic bacteria sensor through an RS485 module. Equipment is automatically controlled to perform liquid preparation treatment in the stem cell culture process, the labor intensity of operators is reduced, and cross contamination is reduced.

Description

technical field [0001] The invention relates to a control system of a liquid dispensing chamber, more specifically a programmable controller-based stem cell liquid dispensing chamber control system and a control method thereof. Background technique [0002] In the process of cell culture, stem cell culture is completed in a petri dish. The bottom of the T175 petri dish is large, the mouth of the bottle is small, and there is a cap with air holes on it; Use a pipette gun to move the seed cell suspension and medium to the culture dish, close the lid, shake well, spread it on the inner surface of the culture dish, and then transfer it to the incubator for culture; when harvesting cells, first remove the Unscrew the lid, pour off the culture medium, add physiological saline to the culture dish through a pipette gun, wash the culture dish and pour out the normal saline, then add trypsin to the culture dish with a pipette gun, and let the culture dish stand horizontally After sev...

AI Technical Summary

Problems solved by technology

[0002] In the process of cell culture, stem cell culture is completed in a petri dish. The bottom of the T175 petri dish is large, the mouth of the bottle is small, and there is a cap with air holes on it; Use a pipette gun to move the seed cell suspension and medium to the culture dish, close the lid, shake well, spread it on the inner surface of the culture dish, and then transfer it to the incubator for culture; when harvesting cells, first remove the Unscrew the lid, pour off the culture medium, add physiological saline to the culture dish through a pipette gun, wash the culture dish and pour out the normal saline, then add trypsin to the culture dish with a pipette gun, and let the culture dish stand horizontally After several minutes, when the cells are peeled off from the inner wall of the culture dish, use a pipette gun to add the digestion termination solution into the culture dish to stop the digestion of the cells, and finally collect the cells in the culture dish and pour them into a centrifuge bottle. Liquid and other operations are all done manually, with many liquid preparation procedures and high labor intensity, and operating a pipette to pipette liquid, unscrewing and tightening the cap of the culture bottle, frequent contact with the culture dish will bring cross-contamination, resulting in cell culture failure

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