Fluorescent biosensor based on nucleic acid recognition induction and application thereof

Abstract

The invention provides a fluorescent biosensor based on nucleic acid recognition induction and an application thereof. The fluorescent biosensor is composed of gold nanoparticles, a recognition sequence and n signal probes, the recognition sequence is single-stranded DNA, the surfaces of the gold nanoparticles are connected with one ends of a plurality of recognition sequences; the signal probe issingle-stranded DNA of which one end is connected with a fluorophore; and the fluorophores of the signal probes are different. The single-stranded DNA sequence of the signal probe is composed of a first DNA sequence and a second DNA sequence. The fluorophore is connected with a second DNA sequence; each recognition sequence connected with the gold nanoparticles is hybridized with the first DNA sequence of the n signal probes; the recognition sequence is complementary with the miRNA-155, and after the recognition sequence is hybridized with the miRNA-155, the 3'end of the recognition sequenceis changed into a blunt end which can be recognized and hydrolyzed by the exonuclease III, and the n signal probes are all unlinked with the recognition sequence; wherein n is a natural number greaterthan 1.

Description

technical field [0001] The disclosure belongs to the technical field of biological detection and relates to a detection method of miRNA-155, in particular to a fluorescent biosensor based on nucleic acid recognition induction and its application. Background technique [0002] The statements herein merely provide background information related to the present disclosure and may not necessarily constitute prior art. [0003] MicroRNA (miRNA), as a class of endogenous non-coding ribonucleic acid containing 18-25 nucleotides, plays an important role in physiological and pathological processes such as cell proliferation, gene expression and metabolism. Many studies have shown that the abnormal expression of miRNA is closely related to many human diseases, especially the development of cancer. There is evidence that aberrant activation of miRNA-155 in blood or cell samples can be affected by cancer. miRNA-155 is expected to be a biomarker for early diagnosis and treatment of canc...

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Problems solved by technology

Although it has been proved that these methods have good sensitivity and selectivity, the inventors of the present disclosure found that these methods for detecting miRNA are slow and costly, which affects and limits their popularization and application

Due to its low cost, simple operation and good reproducibility, the fluorescence detection method can solve these problems such as slow speed and high cost. However, after further research by the inventors of the present disclosure, it is found that the fluorescence detection method has low sensitivity and high accuracy. low level problem

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