Treatment model for osteoarthritis by human adipose-derived stem cells and treatment method thereof
An adipose-derived stem cell and osteoarthritis technology, applied in the field of cell engineering, can solve the problems of insufficient control stability of stem cell therapy, uncontrollable changes in various processes, poor induction and differentiation effect, etc. and differentiation effect, the effect of enhancing the therapeutic effect
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Embodiment 1
[0052] A human-derived adipose stem cell treatment model and treatment method for osteoarthritis, the model treatment method includes the following steps:
[0053] P1. Preparation of stem cell injection: select human-derived adipose tissue, break it up and separate the oil layer to prepare stem cell injection;
[0054] P2. Establishment of osteoarthritis model: Select 6-week-old experimental white mice and inject induction solution into the knee joint cavity for 1 week to induce knee arthritis and establish an osteoarthritis model;
[0055] P3. Model separation: the osteoarthritis model mice were divided into two groups, which were respectively coded as local injection model mice and intravenous injection model mice;
[0056] P4. Intervention treatment: Intervention treatment for local injection of model mice, local injection of stem cell injection at the site of osteoarthritis induction, intravenous injection of stem cell injection for intravenous injection of model mice, and...
Embodiment 2
[0074] A human-derived adipose stem cell treatment model and treatment method for osteoarthritis, the model treatment method includes the following steps:
[0075] P1. Preparation of stem cell injection: select human-derived adipose tissue, break it up and separate the oil layer to prepare stem cell injection;
[0076] P2. Establishment of osteoarthritis model: Select 8-week-old experimental white mice and inject induction solution into the knee joint cavity for 2 weeks to induce knee arthritis and establish an osteoarthritis model;
[0077] P3. Model separation: the osteoarthritis model mice were divided into two groups, which were respectively coded as local injection model mice and intravenous injection model mice;
[0078] P4. Intervention treatment: Intervention treatment for local injection of model mice, local injection of stem cell injection at the induction site of osteoarthritis, intravenous injection of stem cell injection for intravenous injection of model white mi...
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